{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T02:43:02Z","timestamp":1782960182262,"version":"3.54.5"},"reference-count":31,"publisher":"Portland Press Ltd.","issue":"5","license":[{"start":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T00:00:00Z","timestamp":1708300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003554","name":"Lundbeck Foundation","doi-asserted-by":"publisher","award":["R323-2018-3674"],"award-info":[{"award-number":["R323-2018-3674"]}],"id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003554","name":"Lundbeck Foundation","doi-asserted-by":"publisher","award":["R400-2022-1213"],"award-info":[{"award-number":["R400-2022-1213"]}],"id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,3,6]]},"abstract":"<jats:p>The dynamic nature of the microtubule network is dependent in part by post-translational modifications (PTMs) \u2014 particularly through acetylation, which stabilizes the microtubule network. Whether PTMs of the microtubule network in vascular smooth muscle cells (VSMCs) contribute to the pathophysiology of hypertension is unknown. The aim of this study was to determine the acetylated state of the microtubule network in the mesenteric arteries of spontaneously hypertensive rats (SHR). Experiments were performed on male normotensive rats and SHR mesenteric arteries. Western blotting and mass spectrometry determined changes in tubulin acetylation. Wire myography was used to investigate the effect of tubacin on isoprenaline-mediated vasorelaxations. Isolated cells from normotensive rats were used for scanning ion conductance microscopy (SICM). Mass spectrometry and Western blotting showed that tubulin acetylation is increased in the mesenteric arteries of the SHR compared with normotensive rats. Tubacin enhanced the \u03b2-adrenoceptor-mediated vasodilatation by isoprenaline when the endothelium was intact, but attenuated relaxations when the endothelium was denuded or nitric oxide production was inhibited. By pre-treating vessels with colchicine to disrupt the microtubule network, we were able to confirm that the effects of tubacin were microtubule-dependent. Using SICM, we examined the cell surface Young's modulus of VSMCs, but found no difference in control, tubacin-treated, or taxol-treated cells. Acetylation of tubulin at Lys40 is elevated in mesenteric arteries from the SHR. Furthermore, this study shows that tubacin has an endothelial-dependent bimodal effect on isoprenaline-mediated vasorelaxation.<\/jats:p>","DOI":"10.1042\/bcj20230420","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T17:31:46Z","timestamp":1708363906000},"page":"387-403","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mesenteric artery smooth muscle cells from hypertensive rats have increased microtubule acetylation"],"prefix":"10.1042","volume":"481","author":[{"given":"Anthony M.","family":"Mozzicato","sequence":"first","affiliation":[{"name":"1Vascular Biology Group, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joakim A.","family":"Bastrup","sequence":"additional","affiliation":[{"name":"1Vascular Biology Group, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose L.","family":"Sanchez-Alonso","sequence":"additional","affiliation":[{"name":"2Myocardial Function, National Heart and Lung Institute, Imperial College London, ICTEM, Hammersmith Hospital, London, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"van der Horst","sequence":"additional","affiliation":[{"name":"1Vascular Biology Group, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Gorelik","sequence":"additional","affiliation":[{"name":"2Myocardial Function, National Heart and Lung Institute, Imperial College London, ICTEM, Hammersmith Hospital, London, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"H\u00e4gglund","sequence":"additional","affiliation":[{"name":"3Protein Oxidation Group, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9903-714X","authenticated-orcid":false,"given":"Thomas A.","family":"Jepps","sequence":"additional","affiliation":[{"name":"1Vascular Biology Group, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"key":"2024022911503768100_BCJ-2023-0420C1","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1161\/HYPERTENSIONAHA.117.10152","article-title":"Microtubule regulation of Kv7 channels orchestrates cAMP-mediated vasorelaxations in rat arterial smooth muscle","volume":"71","year":"2018","journal-title":"Hypertension"},{"key":"2024022911503768100_BCJ-2023-0420C2","doi-asserted-by":"publisher","first-page":"2214","DOI":"10.1161\/HYPERTENSIONAHA.122.19351","article-title":"Dynein coordinates \u03b22-adrenoceptor-mediated relaxation in normotensive and hypertensive rat mesenteric arteries","volume":"79","year":"2022","journal-title":"Hypertension"},{"key":"2024022911503768100_BCJ-2023-0420C3","doi-asserted-by":"publisher","first-page":"e202012760","DOI":"10.1085\/jgp.202012760","article-title":"Dynein regulates Kv7.4 channel trafficking from the cell membrane","volume":"153","year":"2021","journal-title":"J. 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