{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:37:08Z","timestamp":1753871828441,"version":"3.41.2"},"reference-count":0,"publisher":"Portland Press Ltd.","license":[{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"vor","delay-in-days":32,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000268","name":"UKRI | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["PIII057"],"award-info":[{"award-number":["PIII057"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010221","name":"Higher Education Commision, Pakistan","doi-asserted-by":"publisher","award":["IRSIP 51 BMS 08"],"award-info":[{"award-number":["IRSIP 51 BMS 08"]}],"id":[{"id":"10.13039\/501100010221","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"<jats:p>Cellulosic microfibrils in plant cell walls are largely ensheathed and probably tethered by hydrogen-bonded hemicelluloses. Ensheathing may vary developmentally as hemicelluloses are peeled to enable cell expansion. We characterised a simple method to quantify ensheathed versus naked cellulosic surfaces based on the ability to adsorb a radiolabelled \u2018cellulose-complementary oligosaccharide\u2019, [3H]cellopentaitol. Filter-paper (cellulose) adsorbed 40% and &amp;gt;80% of aqueous 5nM [3H]cellopentaitol within ~1h and ~20h respectively. When [3H]cellopentaitol was rapidly dried onto filter-paper, ~50% of it was desorbable by water, whereas after ~1d annealing in aqueous medium the adsorption became too strong to be reversible in water. \u2018Strongly\u2019 adsorbed [3H]cellopentaitol was, however, ~98% desorbed by 6M NaOH, ~50% by 0.2M cellobiose, and ~30% by 8M urea, indicating a role for hydrogen-bonding reinforced by complementarity of shape. Gradual adsorption was promoted by kosmotropes (1.4M Na2SO4 or 30% methanol), and inhibited by chaotropes (8M urea), supporting a role for hydrogen-bonding. [3H]Cellopentaitol adsorption was strongly competed by non-radioactive cello-oligosaccharides (Cell2\u20136), the IC50 (half-inhibitory concentration) being highly size-dependent: Cell2, ~70 mM; Cell3, ~7 mM; and Cell4-6, ~0.05 mM. Malto-oligosaccharides (400mM) had no effect, confirming the role of complementarity. The quantity of adsorbed [3H]cellopentaitol was proportional to mass of cellulose. Of seven cottons tested, wild-type Gossypium arboreum fibres were least capable of adsorbing [3H]cellopentaitol, indicating ensheathment of their microfibrillar surfaces, confirmed by their resistance to cellulase digestion, and potentially attributable to a high glucuronoarabinoxylan content. In conclusion, [3H]cellopentaitol adsorption is a simple, sensitive and quantitative way of titrating \u2018naked\u2019 cellulose surfaces.<\/jats:p>","DOI":"10.1042\/bcj20240296","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T15:46:18Z","timestamp":1724946378000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Using a cellulose-complementary oligosaccharide as a tool to probe exposed cellulosic surfaces in cotton fibres and growing plant cell walls"],"prefix":"10.1042","author":[{"given":"Mahnoor","family":"Imran","sequence":"first","affiliation":[{"name":"The University of Edinburgh, EDINBURGH, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lenka","family":"Frankov\u00e1","sequence":"additional","affiliation":[{"name":"The University of Edinburgh, EDINBURGH, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uzma","family":"Qaisar","sequence":"additional","affiliation":[{"name":"University of the Punja, Lahore, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen C.","family":"Fry","sequence":"additional","affiliation":[{"name":"The University of Edinburgh, EDINBURGH, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2024,7,28]]},"container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/doi\/10.1042\/BCJ20240296\/960667\/bcj-2024-0296.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/doi\/10.1042\/BCJ20240296\/960667\/bcj-2024-0296.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T15:46:20Z","timestamp":1724946380000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/doi\/10.1042\/BCJ20240296\/234925\/Using-a-cellulose-complementary-oligosaccharide-as"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,28]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1042\/bcj20240296","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"type":"print","value":"0264-6021"},{"type":"electronic","value":"1470-8728"}],"subject":[],"published":{"date-parts":[[2024,7,28]]},"article-number":"BCJ20240296"}}