{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:27:38Z","timestamp":1787128058463,"version":"build-2736575974"},"reference-count":67,"publisher":"Portland Press Ltd.","issue":"9","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32570205"],"award-info":[{"award-number":["32570205"]}]},{"name":"Stavros Niarchos Foundation Institute for Global Infectious Disease Research","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Transcription\u2014the process by which genomic DNA is converted into RNA\u2014is a highly dynamic and tightly controlled process across all domains of life. Although bacteria were once regarded as relatively simple organisms, their transcriptomes are now recognized to be remarkably complex, heterogeneous, and subject to multilayered regulation. Despite the availability of an abundance of sequenced bacterial genomes, a comprehensive understanding of how bacteria tune their transcriptional output to adapt to changing environments remains lacking. To this end, SEnd-seq (simultaneous 5\u2032 and 3\u2032 end sequencing) was developed as a high-throughput approach uniquely capable of simultaneously capturing both 5\u2032 and 3\u2032 ends of individual RNA molecules, enabling the reconstruction of full-length transcripts. By capturing each RNA molecule as a distinct molecular entity with single-nucleotide resolution, SEnd-seq has uncovered previously unrecognized transcriptional features across diverse bacterial species, including even the well-studied Escherichia coli. This method performs robustly across a wide range of RNA species and organisms, including hard-to-lyse pathogens such as Mycobacterium tuberculosis. Moreover, SEnd-seq exhibits high sensitivity for detecting low-abundance RNA and is compatible with various target RNA enrichment strategies, as well as genetic, chemical, and functional perturbations, enabling context-specific transcriptomic analyses. As a versatile and broadly adaptable technology, SEnd-seq provides comprehensive insights into transcriptional regulation, RNA processing, and the coordination between RNA-based processes, thereby uncovering potential targets for antibiotic development. In the present review, we summarize the features and applications of SEnd-seq and discuss its future methodological development and expansion into broader biological and biomedical research contexts.<\/jats:p>","DOI":"10.1042\/bcj20250251","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:09:43Z","timestamp":1787126983000},"page":"1713-1727","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-resolution profiling of bacterial transcriptomes by SEnd-seq"],"prefix":"10.1042","volume":"483","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7831-0899","authenticated-orcid":false,"given":"Xiangwu","family":"Ju","sequence":"first","affiliation":[{"name":"1Laboratory of Bacterial Gene Regulation and Drug Resistance, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4238-7066","authenticated-orcid":false,"given":"Shixin","family":"Liu","sequence":"additional","affiliation":[{"name":"2Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, New York, NY 10065, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"2026081904093924900_B1","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1038\/s41392-022-00974-4","article-title":"Microbiota in health and diseases","volume":"7","author":"Hou","year":"2022","journal-title":"Signal Transduct. Target Ther."},{"key":"2026081904093924900_B2","doi-asserted-by":"publisher","first-page":"e1002533","DOI":"10.1371\/journal.pbio.1002533","article-title":"Revised estimates for the number of human and bacteria cells in the body","volume":"14","author":"Sender","year":"2016","journal-title":"PLoS Biol."},{"key":"2026081904093924900_B3","doi-asserted-by":"crossref","DOI":"10.54394\/CJQU6666","volume-title":"Global tuberculosis report 2024","author":"World Health Organization","year":"2024"},{"key":"2026081904093924900_B4","doi-asserted-by":"publisher","first-page":"1360","DOI":"10.1128\/JB.00935-15","article-title":"Mycobacterium tuberculosis transcription machinery: ready to respond to host attacks","volume":"198","author":"Flentie","year":"2016","journal-title":"J. Bacteriol."},{"key":"2026081904093924900_B5","doi-asserted-by":"publisher","first-page":"4040","DOI":"10.1016\/j.jmb.2019.05.041","article-title":"Transcription of bacterial chromatin","volume":"431","author":"Shen","year":"2019","journal-title":"J. Mol. Biol."},{"key":"2026081904093924900_B6","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.mib.2008.02.016","article-title":"Regulation of bacterial RNA polymerase sigma factor activity: a structural perspective","volume":"11","author":"Campbell","year":"2008","journal-title":"Curr. Opin. Microbiol."},{"key":"2026081904093924900_B7","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1016\/j.molcel.2017.12.023","article-title":"Bacterial RNA biology on a genome scale","volume":"70","author":"Hor","year":"2018","journal-title":"Mol. Cell."},{"key":"2026081904093924900_B8","doi-asserted-by":"publisher","first-page":"e0052422","DOI":"10.1128\/mbio.00524-22","article-title":"Mapping the complex transcriptional landscape of the phytopathogenic bacterium Dickeya dadantii","volume":"13","author":"Forquet","year":"2022","journal-title":"mBio"},{"key":"2026081904093924900_B9","doi-asserted-by":"publisher","first-page":"1907","DOI":"10.1038\/s41564-019-0500-z","article-title":"Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria","volume":"4","author":"Ju","year":"2019","journal-title":"Nat. Microbiol."},{"key":"2026081904093924900_B10","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1038\/s41586-024-07105-9","article-title":"Incomplete transcripts dominate the Mycobacterium tuberculosis transcriptome","volume":"627","author":"Ju","year":"2024","journal-title":"Nature"},{"key":"2026081904093924900_B11","doi-asserted-by":"publisher","first-page":"1359","DOI":"10.1126\/science.abb5317","article-title":"Structural basis of transcription-translation coupling","volume":"369","author":"Wang","year":"2020","journal-title":"Science"},{"key":"2026081904093924900_B12","doi-asserted-by":"publisher","first-page":"2132","DOI":"10.1016\/j.csbj.2024.05.033","article-title":"Genome and transcriptomic analysis of the adaptation of Escherichia coli to environmental stresses","volume":"23","author":"Jiao","year":"2024","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"2026081904093924900_B13","doi-asserted-by":"publisher","first-page":"76","DOI":"10.5808\/GI.2013.11.2.76","article-title":"Current challenges in bacterial transcriptomics","volume":"11","author":"Cho","year":"2013","journal-title":"Genomics Inform."},{"key":"2026081904093924900_B14","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1186\/s12864-025-12094-8","article-title":"Commentary: a review of technical considerations for planning an RNA-Sequencing experiment","volume":"26","author":"Verma","year":"2025","journal-title":"BMC Genomics"},{"key":"2026081904093924900_B15","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1038\/s41576-019-0150-2","article-title":"RNA sequencing: the teenage years","volume":"20","author":"Stark","year":"2019","journal-title":"Nat. Rev. Genet."},{"key":"2026081904093924900_B16","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1186\/s12864-022-08386-y","article-title":"A versatile 5\u2032 RACE-Seq methodology for the accurate identification of the 5\u2032 termini of mRNAs","volume":"23","author":"Adamopoulos","year":"2022","journal-title":"BMC Genomics"},{"key":"2026081904093924900_B17","doi-asserted-by":"publisher","first-page":"aad9822","DOI":"10.1126\/science.aad9822","article-title":"Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria","volume":"352","author":"Dar","year":"2016","journal-title":"Science"},{"key":"2026081904093924900_B18","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.ymeth.2015.06.012","article-title":"Differential RNA-seq (dRNA-seq) for annotation of transcriptional start sites and small RNAs in Helicobacter pylori","volume":"86","author":"Bischler","year":"2015","journal-title":"Methods"},{"key":"2026081904093924900_B19","doi-asserted-by":"publisher","first-page":"gkaf030","DOI":"10.1093\/nar\/gkaf030","article-title":"A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis","volume":"53","author":"Taggart","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"2026081904093924900_B20","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1038\/nature12121","article-title":"Extensive transcriptional heterogeneity revealed by isoform profiling","volume":"497","author":"Pelechano","year":"2013","journal-title":"Nature"},{"key":"2026081904093924900_B21","doi-asserted-by":"publisher","first-page":"1740","DOI":"10.1038\/nprot.2014.121","article-title":"Genome-wide identification of transcript start and end sites by transcript isoform sequencing","volume":"9","author":"Pelechano","year":"2014","journal-title":"Nat. Protoc."},{"key":"2026081904093924900_B22","doi-asserted-by":"publisher","first-page":"3676","DOI":"10.1038\/s41467-018-05997-6","article-title":"SMRT-Cappable-seq reveals complex operon variants in bacteria","volume":"9","author":"Yan","year":"2018","journal-title":"Nat. Commun."},{"key":"2026081904093924900_B23","doi-asserted-by":"publisher","first-page":"20190097","DOI":"10.1098\/rstb.2019.0097","article-title":"Realizing the potential of full-length transcriptome sequencing","volume":"374","author":"Byrne","year":"2019","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"2026081904093924900_B24","doi-asserted-by":"publisher","first-page":"3338","DOI":"10.1016\/j.jmb.2020.03.022","article-title":"Sequencing and structure probing of long rnas using MarathonRT: a next-generation reverse transcriptase","volume":"432","author":"Guo","year":"2020","journal-title":"J. Mol. Biol."},{"key":"2026081904093924900_B25","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1261\/rna.054999.115","article-title":"Adenylylation of small RNA sequencing adapters using the TS2126 RNA ligase I","volume":"22","author":"Lama","year":"2016","journal-title":"RNA"},{"key":"2026081904093924900_B26","doi-asserted-by":"publisher","first-page":"781","DOI":"10.15252\/msb.20145794","article-title":"Genome-wide study of mRNA degradation and transcript elongation in Escherichia coli","volume":"11","author":"Chen","year":"2015","journal-title":"Mol. Syst. Biol."},{"key":"2026081904093924900_B27","doi-asserted-by":"publisher","first-page":"6647597","DOI":"10.1155\/2021\/6647597","article-title":"Bias in RNA-seq library preparation: current challenges and solutions","volume":"2021","author":"Shi","year":"2021","journal-title":"Biomed. Res. Int."},{"key":"2026081904093924900_B28","doi-asserted-by":"publisher","first-page":"8746","DOI":"10.1093\/nar\/gkae601","article-title":"Analysis of bacterial transcriptome and epitranscriptome using nanopore direct RNA sequencing","volume":"52","author":"Tan","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"2026081904093924900_B29","doi-asserted-by":"publisher","first-page":"e31","DOI":"10.1158\/0008-5472.CAN-17-0337","article-title":"Variant Review with the Integrative Genomics Viewer","volume":"77","author":"Robinson","year":"2017","journal-title":"Cancer Res."},{"key":"2026081904093924900_B30","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.mib.2014.11.011","article-title":"Quantitative bacterial transcriptomics with RNA-seq","volume":"23","author":"Creecy","year":"2015","journal-title":"Curr. Opin. Microbiol."},{"key":"2026081904093924900_B31","doi-asserted-by":"publisher","first-page":"5765","DOI":"10.1038\/s41598-022-09710-y","article-title":"Comparison of rRNA depletion methods for efficient bacterial mRNA sequencing","volume":"12","author":"Wahl","year":"2022","journal-title":"Sci. Rep."},{"key":"2026081904093924900_B32","doi-asserted-by":"publisher","first-page":"912","DOI":"10.1186\/1471-2164-15-912","article-title":"Comparison of library preparation methods reveals their impact on interpretation of metatranscriptomic data","volume":"15","author":"Alberti","year":"2014","journal-title":"BMC Genomics"},{"key":"2026081904093924900_B33","doi-asserted-by":"publisher","first-page":"4424","DOI":"10.1093\/bioinformatics\/btab515","article-title":"tsRNAsearch: a pipeline for the identification of tRNA and ncRNA fragments from small RNA-sequencing data","volume":"37","author":"Donovan","year":"2021","journal-title":"Bioinformatics"},{"key":"2026081904093924900_B34","doi-asserted-by":"publisher","first-page":"1934","DOI":"10.1074\/jbc.M116.761171","article-title":"Identification of the RNA pyrophosphohydrolase RppH of Helicobacter pylori and global analysis of its RNA targets","volume":"292","author":"Bischler","year":"2017","journal-title":"J. Biol. Chem."},{"key":"2026081904093924900_B35","doi-asserted-by":"publisher","first-page":"5038","DOI":"10.1074\/jbc.M115.702555","article-title":"Distinct requirements for 5\u2032-monophosphate-assisted RNA cleavage by Escherichia coli RNase E and RNase G","volume":"291","author":"Richards","year":"2016","journal-title":"J. Biol. Chem."},{"key":"2026081904093924900_B36","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.molcel.2007.05.038","article-title":"Initiation of RNA decay in Escherichia coli by 5\u2032 pyrophosphate removal","volume":"27","author":"Celesnik","year":"2007","journal-title":"Mol. Cell."},{"key":"2026081904093924900_B37","doi-asserted-by":"publisher","first-page":"1400","DOI":"10.1016\/j.cell.2015.05.008","article-title":"Widespread co-translational RNA decay reveals ribosome dynamics","volume":"161","author":"Pelechano","year":"2015","journal-title":"Cell"},{"key":"2026081904093924900_B38","doi-asserted-by":"publisher","first-page":"906","DOI":"10.1038\/s41467-021-21150-2","article-title":"Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria","volume":"12","author":"Sun","year":"2021","journal-title":"Nat. Commun."},{"key":"2026081904093924900_B39","doi-asserted-by":"publisher","first-page":"3931","DOI":"10.1038\/s41467-023-39576-1","article-title":"Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogen Borrelia burgdorferi","volume":"14","author":"Petroni","year":"2023","journal-title":"Nat. Commun."},{"key":"2026081904093924900_B40","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1038\/s41576-020-0254-8","article-title":"Redefining fundamental concepts of transcription initiation in bacteria","volume":"21","author":"Mejia-Almonte","year":"2020","journal-title":"Nat. Rev. Genet."},{"key":"2026081904093924900_B41","doi-asserted-by":"publisher","first-page":"gkaf1310","DOI":"10.1093\/nar\/gkaf1310","article-title":"Unraveling the start element and regulatory divergence of core promoters across the domain bacteria","volume":"53","author":"Kuo","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"2026081904093924900_B42","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1111\/j.1742-4658.2009.07479.x","article-title":"The sigma factors of Mycobacterium tuberculosis: regulation of the regulators","volume":"277","author":"Sachdeva","year":"2010","journal-title":"FEBS J."},{"key":"2026081904093924900_B43","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.3390\/molecules22071169","article-title":"Co-transcriptional folding and regulation mechanisms of riboswitches","volume":"22","author":"Gong","year":"2017","journal-title":"Molecules"},{"key":"2026081904093924900_B44","doi-asserted-by":"publisher","first-page":"108478","DOI":"10.1016\/j.biotechadv.2024.108478","article-title":"Bacterial 5\u2032 UTR: a treasure-trove for post-transcriptional regulation","volume":"78","author":"Liu","year":"2025","journal-title":"Biotechnol. Adv."},{"key":"2026081904093924900_B45","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1016\/j.jmb.2011.03.036","article-title":"Bacterial transcription terminators: the RNA 3\u2032-end chronicles","volume":"412","author":"Peters","year":"2011","journal-title":"J. Mol. Biol."},{"key":"2026081904093924900_B46","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1146\/annurev-biochem-060815-014844","article-title":"Mechanisms of bacterial transcription termination: all good things must end","volume":"85","author":"Ray-Soni","year":"2016","journal-title":"Annu. Rev. Biochem."},{"key":"2026081904093924900_B47","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1016\/j.molcel.2023.02.017","article-title":"Head-on and co-directional RNA polymerase collisions orchestrate bidirectional transcription termination","volume":"83","author":"Wang","year":"2023","journal-title":"Mol. Cell."},{"key":"2026081904093924900_B48","doi-asserted-by":"publisher","DOI":"10.64898\/2026.04.06.716678","article-title":"Structural principles of transcriptional collisions","author":"Watters","year":"2026","journal-title":"bioRxiv."},{"key":"2026081904093924900_B49","doi-asserted-by":"publisher","first-page":"3627","DOI":"10.1016\/j.molcel.2024.08.021","article-title":"An RNA-centric view of transcription and genome organization","volume":"84","author":"Henninger","year":"2024","journal-title":"Mol. Cell."},{"key":"2026081904093924900_B50","doi-asserted-by":"publisher","first-page":"1920","DOI":"10.1038\/s41564-026-02374-8","article-title":"Spatial and genetic constraints govern coupling between transcription, translation, and mRNA degradation in bacteria","volume":"11","author":"Kim","year":"2026","journal-title":"Nat. Microbiol."},{"key":"2026081904093924900_B51","doi-asserted-by":"publisher","first-page":"2376","DOI":"10.1097\/MS9.0000000000001859","article-title":"New option: targeting RNase J and RNase HI in the fight against multi-drug-resistant tuberculosis","volume":"86","author":"Khadka","year":"2024","journal-title":"Ann. Med. Surg. (Lond.)"},{"key":"2026081904093924900_B52","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1016\/j.molcel.2011.08.022","article-title":"Regulation by small RNAs in bacteria: expanding frontiers","volume":"43","author":"Storz","year":"2011","journal-title":"Mol. Cell."},{"key":"2026081904093924900_B53","doi-asserted-by":"publisher","first-page":"e1461","DOI":"10.1002\/wrna.1461","article-title":"Natural antisense transcripts in diseases: from modes of action to targeted therapies","volume":"9","author":"Wanowska","year":"2018","journal-title":"Wiley Interdiscip Rev. RNA"},{"key":"2026081904093924900_B54","doi-asserted-by":"publisher","first-page":"1246","DOI":"10.1016\/j.cell.2015.05.043","article-title":"Dying mRNA tells a story of its life","volume":"161","author":"Wallace","year":"2015","journal-title":"Cell"},{"key":"2026081904093924900_B55","doi-asserted-by":"publisher","first-page":"2347","DOI":"10.1038\/s41564-019-0543-1","article-title":"Disruption of transcription-translation coordination in Escherichia coli leads to premature transcriptional termination","volume":"4","author":"Zhu","year":"2019","journal-title":"Nat. Microbiol."},{"key":"2026081904093924900_B56","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1038\/s41586-020-2638-5","article-title":"Functionally uncoupled transcription-translation in Bacillus subtilis","volume":"585","author":"Johnson","year":"2020","journal-title":"Nature"},{"key":"2026081904093924900_B57","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1126\/science.abb3758","article-title":"In-cell architecture of an actively transcribing-translating expressome","volume":"369","author":"O'Reilly","year":"2020","journal-title":"Science"},{"key":"2026081904093924900_B58","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1146\/annurev-genet-072220-033342","article-title":"Transcription-translation coupling in bacteria","volume":"56","author":"Blaha","year":"2022","journal-title":"Annu. Rev. Genet."},{"key":"2026081904093924900_B59","doi-asserted-by":"publisher","first-page":"e102500","DOI":"10.15252\/embj.2019102500","article-title":"The torpedo effect in Bacillus subtilis: RNase J1 resolves stalled transcription complexes","volume":"39","author":"Sikova","year":"2020","journal-title":"EMBO J."},{"key":"2026081904093924900_B60","doi-asserted-by":"publisher","first-page":"150225","DOI":"10.1016\/j.bbrc.2024.150225","article-title":"Machine learning and related approaches in transcriptomics","volume":"724","author":"Cheng","year":"2024","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026081904093924900_B61","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1038\/s41579-023-00984-1","article-title":"Machine learning for microbiologists","volume":"22","author":"Asnicar","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"2026081904093924900_B62","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1038\/s41580-021-00407-0","article-title":"A guide to machine learning for biologists","volume":"23","author":"Greener","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2026081904093924900_B63","doi-asserted-by":"publisher","first-page":"e00439","DOI":"10.1128\/mSystems.00439-20","article-title":"Benchmarking bacterial promoter prediction tools: potentialities and limitations","volume":"5","author":"Cassiano","year":"2020","journal-title":"mSystems"},{"key":"2026081904093924900_B64","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.jbiosc.2025.01.008","article-title":"Recent advances in single-cell RNA sequencing of bacteria: techniques, challenges, and applications","volume":"139","author":"Nishimura","year":"2025","journal-title":"J. Biosci. Bioeng."},{"key":"2026081904093924900_B65","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1038\/s41580-022-00566-8","article-title":"Long non-coding RNAs: definitions, functions, challenges and recommendations","volume":"24","author":"Mattick","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2026081904093924900_B66","doi-asserted-by":"publisher","first-page":"e0020624","DOI":"10.1128\/msystems.00206-24","article-title":"Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host\u2013pathogen interaction","volume":"9","author":"Hu","year":"2024","journal-title":"mSystems"},{"key":"2026081904093924900_B67","doi-asserted-by":"publisher","first-page":"D687","DOI":"10.1093\/nar\/gkaa890","article-title":"DualSeqDB: the host\u2013pathogen dual RNA sequencing database for infection processes","volume":"49","author":"Macho Rendon","year":"2021","journal-title":"Nucleic Acids Res."}],"container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/483\/9\/1713\/991034\/bcj-2025-0251c.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/483\/9\/1713\/991034\/bcj-2025-0251c.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:09:44Z","timestamp":1787126984000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/483\/9\/1713\/237935\/High-resolution-profiling-of-bacterial"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":67,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,2]]}},"URL":"https:\/\/doi.org\/10.1042\/bcj20250251","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,8,19]]}}}