{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T21:31:16Z","timestamp":1767907876983,"version":"3.49.0"},"reference-count":41,"publisher":"Portland Press Ltd.","issue":"01","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":15,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,1,7]]},"abstract":"<jats:p>Using Nakaseomyces glabrata as a model organism, we demonstrate that targeting calcineurin can synergize with caspofungin to induce a quorum sensing (QS) effect mediated by farnesol. This QS effect requires calcineurin deficiency, sub-minimum inhibitory concentration (MIC) levels of caspofungin, and a high-density cell population. Cell growth and biofilm formation were significantly inhibited within a specific range of cell density and sub-MIC caspofungin treatment in the calcineurin mutant. The inhibition of biofilm formation follows the \u2018paradoxical growth,\u2019 showing a concentration-dependent response to caspofungin. We show that high cell density triggers two antagonistic effects: overcoming antibiotic inhibition, which promotes cell propagation, and QS-mediated growth inhibition, which negatively regulates cell proliferation. The QS molecule farnesol was detectable only in the calcineurin mutant, where the transcription of the farnesol synthase Dpp3 was significantly up-regulated, and deletion of DPP3 abolished the QS effect in both spot assay and biofilm formation of the calcineurin mutant. Besides this, we identified a Dpp3-dependent, ergosterol-farnesol metabolism-linked Crz1-independent regulatory mechanism that contributes to the calcineurin-mediated multi-stress resistance. We demonstrate that calcineurin, Dpp3, and caspofungin are all involved in regulating ergosterol metabolism and the transcription of ERG11 and FKS genes, leading to significant changes in membrane and cell wall stress tolerance. The cell wall composition undergoes substantial alterations upon deletion of calcineurin or treatment with caspofungin, while caspofungin also increases the levels of \u03b2-glucan and short peptides in the medium, tentatively pointing to the release of QS inducers from the cell wall.<\/jats:p>","DOI":"10.1042\/bcj20253193","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T18:52:11Z","timestamp":1766429531000},"page":"37-54","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting calcineurin: a synergistic strategy with caspofungin to induce farnesol-mediated quorum sensing in\n                    <i>Nakaseomyces glabrata<\/i>\n                    \u2014an inoculum-dependent tale"],"prefix":"10.1042","volume":"483","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2735-3055","authenticated-orcid":false,"given":"Yu-Ke","family":"Cen","sequence":"first","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu-Lu","family":"Zhang","sequence":"additional","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi-Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao-Xu","family":"Lu","sequence":"additional","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan-Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Xiang","sequence":"additional","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0370-2502","authenticated-orcid":false,"given":"Ya-Ping","family":"Xue","sequence":"additional","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6358-6243","authenticated-orcid":false,"given":"Yu-Guo","family":"Zheng","sequence":"additional","affiliation":[{"name":"1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"2Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, China"},{"name":"3National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"2026010812030068200_BCJ-2025-3193C1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1021\/acsinfecdis.4c00477","article-title":"Candida glabrata: a tale of stealth and endurance","volume":"11","author":"Askari","year":"2025","journal-title":"ACS Infect. Dis."},{"key":"2026010812030068200_BCJ-2025-3193C2","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41572-024-00503-3","article-title":"Invasive candidiasis","volume":"10","author":"Lass-Fl\u00f6rl","year":"2024","journal-title":"Nat. Rev. Dis. Primers"},{"key":"2026010812030068200_BCJ-2025-3193C3","doi-asserted-by":"crossref","first-page":"2954","DOI":"10.1038\/s41564-024-01811-w","article-title":"Collateral sensitivity counteracts the evolution of antifungal drug resistance in Candida auris","volume":"9","author":"Carolus","year":"2024","journal-title":"Nat. Microbiol."},{"key":"2026010812030068200_BCJ-2025-3193C4","doi-asserted-by":"crossref","first-page":"S79","DOI":"10.1093\/ofid\/ofy358","article-title":"Twenty years of the SENTRY antifungal surveillance program: results for Candida species from 1997-2016","volume":"6","author":"Pfaller","year":"2019","journal-title":"Open Forum Infect. Dis."},{"key":"2026010812030068200_BCJ-2025-3193C5","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1128\/AAC.01397-07","article-title":"Synergistic effect of calcineurin inhibitors and fluconazole against Candida albicans biofilms","volume":"52","author":"Uppuluri","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"2026010812030068200_BCJ-2025-3193C6","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1534\/g3.112.002279","article-title":"Convergent evolution of calcineurin pathway roles in thermotolerance and virulence in Candida glabrata","volume":"2","author":"Chen","year":"2012","journal-title":"G3 (Bethesda, Md.)"},{"key":"2026010812030068200_BCJ-2025-3193C7","doi-asserted-by":"crossref","first-page":"126786","DOI":"10.1016\/j.micres.2021.126786","article-title":"Calcium-calcineurin signaling pathway in Candida albicans: a potential drug target","volume":"249","author":"Li","year":"2021","journal-title":"Microbiol. Res."},{"key":"2026010812030068200_BCJ-2025-3193C8","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1038\/nature07292","article-title":"Frequency-modulated nuclear localization bursts coordinate gene regulation","volume":"455","author":"Cai","year":"2008","journal-title":"Nature New Biol."},{"key":"2026010812030068200_BCJ-2025-3193C9","volume-title":"Ontwikkeling van Nieuwe Moleculaire Technieken En Onderzoek Naar de Gpr1 En Calcineurine-Afhankelijke Regulatie van Stressresistentie in Candida Glabrata","author":"Cen","year":"2016"},{"key":"2026010812030068200_BCJ-2025-3193C10","doi-asserted-by":"crossref","first-page":"e00986-15","DOI":"10.1128\/mBio.00986-15","article-title":"Cell wall remodeling enzymes modulate fungal cell wall elasticity and osmotic stress resistance","volume":"6","author":"Ene","year":"2015","journal-title":"MBio"},{"key":"2026010812030068200_BCJ-2025-3193C11","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1186\/s12964-023-01224-y","article-title":"A glycosylated Phr1 protein is induced by calcium stress and its expression is positively controlled by the calcium\/calcineurin signaling transcription factor Crz1 in Candida albicans","volume":"21","author":"Jiang","year":"2023","journal-title":"Cell Commun. Signal"},{"key":"2026010812030068200_BCJ-2025-3193C12","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1080\/21505594.2022.2081291","article-title":"Biomarkers of caspofungin resistance in Candida albicans isolates: A proteomic approach","volume":"13","author":"De Cesare","year":"2022","journal-title":"Virulence"},{"key":"2026010812030068200_BCJ-2025-3193C13","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1111\/1469-0691.12039","article-title":"ESCMID* guideline for the diagnosis and management of Candida diseases 2012: non-neutropenic adult patients","volume":"18 Suppl 7","author":"Cornely","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"2026010812030068200_BCJ-2025-3193C14","doi-asserted-by":"crossref","first-page":"e03225-19","DOI":"10.1128\/mBio.03225-19","article-title":"Phosphoric metabolites link phosphate import and polysaccharide biosynthesis for Candida albicans cell wall maintenance","volume":"11","author":"Liu","year":"2020","journal-title":"MBio"},{"key":"2026010812030068200_BCJ-2025-3193C15","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1016\/j.cell.2018.08.003","article-title":"SnapShot: bacterial quorum sensing","volume":"174","author":"Eickhoff","year":"2018","journal-title":"Cell"},{"key":"2026010812030068200_BCJ-2025-3193C16","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/jb.176.2.269-275.1994","article-title":"Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators","volume":"176","author":"Fuqua","year":"1994","journal-title":"J. Bacteriol."},{"key":"2026010812030068200_BCJ-2025-3193C17","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1098\/rstb.2007.2039","article-title":"Look who\u2019s talking: communication and quorum sensing in the bacterial world","volume":"362","author":"Williams","year":"2007","journal-title":"Philos. Trans. R. Soc. Lond., B, Biol. Sci."},{"key":"2026010812030068200_BCJ-2025-3193C18","doi-asserted-by":"crossref","first-page":"2982","DOI":"10.1128\/AEM.67.7.2982-2992.2001","article-title":"Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol","volume":"67","author":"Hornby","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"2026010812030068200_BCJ-2025-3193C19","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1128\/AAC.49.2.584-589.2005","article-title":"cDNA microarray analysis of differential gene expression in Candida albicans biofilm exposed to farnesol","volume":"49","author":"Cao","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"2026010812030068200_BCJ-2025-3193C20","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1128\/AAC.01551-08","article-title":"Farnesol-induced apoptosis in Candida albicans","volume":"53","author":"Shirtliff","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"2026010812030068200_BCJ-2025-3193C21","doi-asserted-by":"crossref","first-page":"D1670","DOI":"10.1093\/nar\/gkae973","article-title":"Generic Diagramming Platform (GDP): a comprehensive database of high-quality biomedical graphics","volume":"53","author":"Jiang","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"2026010812030068200_BCJ-2025-3193C22","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.tim.2018.10.007","article-title":"The eagle effect and antibiotic-induced persistence: two sides of the same coin?","volume":"27","author":"Prasetyoputri","year":"2019","journal-title":"Trends Microbiol."},{"key":"2026010812030068200_BCJ-2025-3193C23","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.3390\/molecules24101950","article-title":"Fungal quorum-sensing molecules and inhibitors with potential antifungal activity: a review","volume":"24","author":"Mehmood","year":"2019","journal-title":"Molecules"},{"key":"2026010812030068200_BCJ-2025-3193C24","doi-asserted-by":"crossref","first-page":"W194","DOI":"10.1093\/nar\/gkae415","article-title":"CASTpFold: computed atlas of surface topography of the universe of protein folds","volume":"52","author":"Ye","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"2026010812030068200_BCJ-2025-3193C25","doi-asserted-by":"crossref","first-page":"e00554-23","DOI":"10.1128\/msphere.00554-23","article-title":"Calcineurin-dependent contributions to fitness in the opportunistic pathogen Candida glabrata","volume":"9","author":"Pavesic","year":"2024","journal-title":"mSphere"},{"key":"2026010812030068200_BCJ-2025-3193C26","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1080\/21505594.2016.1201250","article-title":"Calcineurin in fungal virulence and drug resistance: prospects for harnessing targeted inhibition of calcineurin for an antifungal therapeutic approach","volume":"8","author":"Juvvadi","year":"2017","journal-title":"Virulence"},{"key":"2026010812030068200_BCJ-2025-3193C27","doi-asserted-by":"crossref","first-page":"e1011445","DOI":"10.1371\/journal.ppat.1011445","article-title":"Calcineurin: The Achilles\u2019 heel of fungal pathogens","volume":"19","author":"Yadav","year":"2023","journal-title":"PLoS Pathog."},{"key":"2026010812030068200_BCJ-2025-3193C28","first-page":"488","article-title":"Effect of farnesol on glucan-related genes in Candida albicans biofilm and the drug resistance of Candida albicans","volume":"43","author":"Zhang","year":"2023","journal-title":"Stomatology"},{"key":"2026010812030068200_BCJ-2025-3193C29","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.foodchem.2014.04.086","article-title":"Enzymatic process for the fractionation of baker\u2019s yeast cell wall (Saccharomyces cerevisiae)","volume":"163","author":"Borchani","year":"2014","journal-title":"Food Chem."},{"key":"2026010812030068200_BCJ-2025-3193C30","doi-asserted-by":"crossref","first-page":"441","DOI":"10.3390\/antibiotics12030441","article-title":"Effect of quorum sensing molecule farnesol on mixed biofilms of Candida albicans and Staphylococcus aureus","volume":"12","author":"Ga\u00e1lov\u00e1-Radochov\u00e1","year":"2023","journal-title":"Antibiotics (Basel)."},{"key":"2026010812030068200_BCJ-2025-3193C31","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3390\/jof6030099","article-title":"Fungal quorum-sensing molecules: a review of their antifungal effect against Candida Biofilms","volume":"6","author":"Kov\u00e1cs","year":"2020","journal-title":"J. Fungi (Basel)."},{"key":"2026010812030068200_BCJ-2025-3193C32","doi-asserted-by":"crossref","first-page":"4275","DOI":"10.1038\/s41467-019-12199-1","article-title":"Harnessing calcineurin-FK506-FKBP12 crystal structures from invasive fungal pathogens to develop antifungal agents","volume":"10","author":"Juvvadi","year":"2019","journal-title":"Nat. Commun."},{"key":"2026010812030068200_BCJ-2025-3193C33","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.2174\/1568026619666181210124159","article-title":"Chemistry and biology of farnesol and its derivatives: quorum sensing molecules with immense therapeutic potential","volume":"18","author":"Gupta","year":"2018","journal-title":"Curr. Top. Med. Chem."},{"key":"2026010812030068200_BCJ-2025-3193C34","doi-asserted-by":"crossref","first-page":"e00554-23","DOI":"10.1128\/msphere.00554-23","article-title":"Calcineurin-dependent contributions to fitness in the opportunistic pathogen Candida glabrata","volume":"9","author":"Pavesic","year":"2024","journal-title":"mSphere"},{"key":"2026010812030068200_BCJ-2025-3193C35","doi-asserted-by":"crossref","first-page":"e1006832","DOI":"10.1371\/journal.pgen.1006832","article-title":"The Arabidopsis leucine-rich repeat receptor kinase MIK2\/LRR-KISS connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses","volume":"13","author":"Van der Does","year":"2017","journal-title":"PLoS Genet."},{"key":"2026010812030068200_BCJ-2025-3193C36","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1021\/acs.biochem.2c00305","article-title":"Purification and characterization of a stable, membrane-associated peptidoglycan responsive adenylate cyclase LRR domain from human commensal Candida albicans","volume":"61","author":"Crump","year":"2022","journal-title":"Biochemistry"},{"key":"2026010812030068200_BCJ-2025-3193C37","doi-asserted-by":"crossref","first-page":"e00069-19","DOI":"10.1128\/CMR.00069-19","article-title":"Antifungal susceptibility testing: current approaches","volume":"33","author":"Berkow","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"2026010812030068200_BCJ-2025-3193C38","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1128\/AAC.01364-09","article-title":"Roles of calcineurin and Crz1 in antifungal susceptibility and virulence of Candida glabrata","volume":"54","author":"Miyazaki","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"2026010812030068200_BCJ-2025-3193C39","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1128\/EC.00281-14","article-title":"Deletion of the DNA ligase IV gene in Candida glabrata significantly increases gene-targeting efficiency","volume":"14","author":"Cen","year":"2015","journal-title":"Eukaryotic Cell"},{"key":"2026010812030068200_BCJ-2025-3193C40","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.fgb.2017.08.004","article-title":"Comparison of genome engineering using the CRISPR-Cas9 system in C. glabrata wild-type and lig4 strains","volume":"107","author":"Cen","year":"2017","journal-title":"Fungal Genet. Biol."},{"key":"2026010812030068200_BCJ-2025-3193C41","doi-asserted-by":"crossref","first-page":"3304","DOI":"10.1038\/s41564-024-01854-z","article-title":"Acquired amphotericin B resistance leads to fitness trade-offs that can be mitigated by compensatory evolution in Candida auris","volume":"9","author":"Carolus","year":"2024","journal-title":"Nat. Microbiol."}],"container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/483\/01\/37\/982416\/bcj-2025-3193.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/483\/01\/37\/982416\/bcj-2025-3193.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T17:03:12Z","timestamp":1767891792000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/483\/01\/37\/236987\/Targeting-calcineurin-a-synergistic-strategy-with"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,24]]},"references-count":41,"journal-issue":{"issue":"01","published-print":{"date-parts":[[2026,1,7]]}},"URL":"https:\/\/doi.org\/10.1042\/bcj20253193","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,1]]},"published":{"date-parts":[[2025,12,24]]}}}