{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:52:15Z","timestamp":1792785135548,"version":"4.5.0"},"reference-count":52,"publisher":"Portland Press Ltd.","issue":"2","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000837","name":"University of Nottingham","doi-asserted-by":"crossref","award":["Nottingham Research Felllowship"],"award-info":[{"award-number":["Nottingham Research Felllowship"]}],"id":[{"id":"10.13039\/501100000837","id-type":"DOI","asserted-by":"crossref"}]},{"name":"iuss pavia","award":["PhD SDC"],"award-info":[{"award-number":["PhD SDC"]}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,2,4]]},"abstract":"<jats:p>The production of biofuels by bacterial fermentation receives sustained attention due to the need to develop novel circular and sustainable technologies. Clostridium beijerinckii produces both hydrogen (H2) and carbon-based biofuels acetone, butanol and ethanol (ABE solvents). H2 metabolism in C. beijerinckii is complex and mostly unexplored. Seven hydrogenase genes are contained in the genome, but their exact physiological role is unknown. Here, we report on the characterisation of a novel heterotetrameric soluble enzyme complex composed of an [FeFe]-hydrogenase component stably bound to a formate dehydrogenase subunit, which we name CbFdh\/Hyd. We show that the four subunits form a stable complex that can be conveniently overexpressed and purified recombinantly. CbFdh\/Hyd is highly sensitive to atmospheric oxygen and displays reversible catalytic features, including H2 evolution, H2 uptake, formate oxidation and the ability to split formate into H2 and CO2 (formate hydrogen lyase activity, FHL) as well as the opposite reaction, H2-driven CO2 reduction (HDCR). CbFdh\/Hyd displays functional and spectroscopic features very similar to Fdh\/Hyd complexes previously described in acetogens, suggesting that this enzyme is at the basis of the previously reported unconventional ability of C. beijerinckii to fix CO2 into acetate and butyrate. CbFdh\/Hyd could also represent a key player in H2 production metabolism by degrading formate produced from the decarboxylation of pyruvate.<\/jats:p>","DOI":"10.1042\/bcj20253323","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T15:07:58Z","timestamp":1769180878000},"page":"177-189","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Clostridium beijerinckii<\/i>\n                    displays a soluble [FeFe]-hydrogenase\/formate dehydrogenase enzyme complex that links H2 and CO2 metabolism"],"prefix":"10.1042","volume":"483","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7730-8247","authenticated-orcid":false,"given":"Sabrina","family":"Dezzani","sequence":"first","affiliation":[{"name":"1Faculty of Engineering, University of Nottingham, Nottingham, U.K."},{"name":"2Department of Life Sciences and Systems Biology, University of Turin, Italy"},{"name":"3University School for Advanced Studies IUSS Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8464-3748","authenticated-orcid":false,"given":"Abdulrahman","family":"Alogaidi","sequence":"additional","affiliation":[{"name":"1Faculty of Engineering, University of Nottingham, Nottingham, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8453-0743","authenticated-orcid":false,"given":"Anca","family":"Pordea","sequence":"additional","affiliation":[{"name":"1Faculty of Engineering, University of Nottingham, Nottingham, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1341-191X","authenticated-orcid":false,"given":"Simone","family":"Morra","sequence":"additional","affiliation":[{"name":"1Faculty of Engineering, University of Nottingham, Nottingham, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"2026020411420941600_BCJ-2025-3323C1","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1038\/s41598-018-37679-0","article-title":"Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level","volume":"9","author":"Patakova","year":"2019","journal-title":"Sci. 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