{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,25]],"date-time":"2023-12-25T03:11:42Z","timestamp":1703473902069},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1968,2,1]]},"abstract":"<jats:p>1. Species of Pseudomonas capable of degrading arylsulphonates and detergents of the alkylbenzenesulphonate type were isolated from sewage and river water. 2. Benzenesulphinate, benzenesulphonate and toluene-p-sulphonate were rapidly degraded by these organisms with the release of the sulphonate group as sulphite; detergent homologues with a chain length up to 16 carbon atoms (4-n-hexadecyl-benzenesulphonate) also released sulphite. Sulphite oxidation to sulphate in the medium can occur non-enzymically. 3. Growth on benzenesulphonate and toluene-p-sulphonate elicited a catechol 2,3-oxygenase, which effected a \u2018meta\u2019 cleavage of the ring. The metabolic route for benzenesulphonate was determined as: benzenesulphonate\u2192catechol\u21922-hydroxymuconic semialdehyde\u2192formate and 4-hydroxy-2-oxovalerate\u2192acetaldehyde and pyruvate; the enzymes catalysing these steps were all inducible. 4. Toluene-p-sulphonate was degraded via 2-hydroxy-5-methylmuconic semialdehyde to formate and 4-hydroxy-2-oxohexanoate and the latter was cleaved to propionaldehyde and pyruvate. Propionaldehyde and propionate were oxidized rapidly by toluene-p-sulphonate-grown cells but slowly by fumarate-grown organisms. 5. The specificity of the catechol 2,3-oxygenase induced by the arylsulphonates, towards catechol and the methylcatechols, varied during the purification and suggested that 3-methylcatechol was probably oxidized by a separate enzyme. Detergents of the alkylbenzenesulphonate type also induced a catechol 2,3-oxygenase in these bacteria. 6. A few isolates, after growth on benzenesulphonate, opened the ring of catechol by an \u2018ortho\u2019 route to form cis\u2013cis-muconate. The enzymes to degrade this intermediate to \u03b2-oxoadipate were also present in induced cells.<\/jats:p>","DOI":"10.1042\/bj1060859","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:13:27Z","timestamp":1439234007000},"page":"859-877","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Metabolism of arylsulphonates by micro-organisms"],"prefix":"10.1042","volume":"106","author":[{"given":"R B","family":"Cain","sequence":"first","affiliation":[{"name":"Microbiology Group, Department of Botany, University of Newcastle upon Tyne"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. R.","family":"Farr","sequence":"additional","affiliation":[{"name":"Microbiology Group, Department of Botany, University of Newcastle upon Tyne"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/106\/4\/859\/760580\/bj1060859.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/106\/4\/859\/760580\/bj1060859.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T20:41:26Z","timestamp":1638218486000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/106\/4\/859\/3169\/Metabolism-of-arylsulphonates-by-micro-organisms"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,2,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1968,2,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1060859","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1968,2,1]]}}}