{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T23:58:13Z","timestamp":1678924693226},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1968,12,1]]},"abstract":"<jats:p>The urinary protein excreted in heavy-chain disease was separated by ion-exchange chromatography into two broad fractions designated Cra-1 and Cra-2. For a dimeric molecular weight of approx. 51000, Cra-1 contained 3\u20134 residues of 6-deoxy-l-galactose (l-fucose), 10 of d-mannose, 5\u20136 of d-galactose, 12 of 2-acetamido-2-deoxy-d-glucose (N-acetyl-d-glucosamine) and 4\u20135 of N-acetylneuraminic acid (sialic acid), whereas the corresponding values for Cra-2 were 2, 10, 7, 12 and 7. Cra-2 contained in addition 1 residue of 2-acetamido-2-deoxy-d-galactose (N-acetyl-d-galactosamine). Cra-1 contained an average of four oligosaccharide units, two of which contained 1 residue of 6-deoxy-l-galactose, 3 of d-mannose, 1 of d-galactose and 3 of 2-acetamido-2-deoxy-d-glucose, whereas the other two units contained the same proportions of 6-deoxy-l-galactose, d-mannose and 2-acetamido-2-deoxy-d-glucose but 2 residues of d-galactose and 2 of N-acetylneuraminic acid. Cra-2 also contained an average of four oligosaccharide units, but the range of glycopeptides was much wider, containing 0\u20131 residue of 6-deoxy-l-galactose, 2\u20133 of d-mannose, 2\u20133 of d-galactose, 2\u20133 of 2-acetamido-2-deoxy-d-glucose and 1\u20133 of N-acetylneuraminic acid. Possible reasons for this heterogeneity are discussed. Glycopeptides were also isolated from Cra-2 that contained 1 residue of d-mannose, 2 of d-galactose, 1 of 2-acetamido-2-deoxy-d-galactose and 0\u20133 of N-acetylneuraminic acid.<\/jats:p>","DOI":"10.1042\/bj1100385","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:37:20Z","timestamp":1439501840000},"page":"385-393","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Studies on the glycopeptides isolated from the urinary protein in heavy-chain disease"],"prefix":"10.1042","volume":"110","author":[{"given":"J. R.","family":"Clamp","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Bristol, and Department of Medicine, New York University Medical Center, New York, N.Y. 10016, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Dawson","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Bristol, and Department of Medicine, New York University Medical Center, New York, N.Y. 10016, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. C.","family":"Franklin","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Bristol, and Department of Medicine, New York University Medical Center, New York, N.Y. 10016, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/110\/3\/385\/762158\/bj1100385.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/110\/3\/385\/762158\/bj1100385.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T21:06:48Z","timestamp":1638220008000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/110\/3\/385\/16141\/Studies-on-the-glycopeptides-isolated-from-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,12,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1968,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1100385","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1968,12,1]]}}}