{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:58:33Z","timestamp":1791316713771,"version":"4.3.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1968,12,1]]},"abstract":"<jats:p>1. Pneumococcal C-substance was isolated from the non-capsulated Pneumococcus 1\u2013192R, A.T.C.C. 12213, by extraction with trichloroacetic acid solution followed by chromatography on DEAE-cellulose (HCO3\u2212 form). 2. The polymer contains 7\u00b70% of phosphorus and 6\u00b70% of nitrogen and is composed of phosphate, N-acetyl-d-galactosamine, d-glucose, N-acetyldiaminotrideoxyhexose, ribitol and choline in the molecular proportions 2:1:1:1:1:1. 3. After acid hydrolysis, d-galactosamine hydrochloride and galactosamine 6-phosphate were isolated in crystalline form and crystalline derivatives of d-glucose and anhydroribitol were obtained. A product of partial acid hydrolysis was provisionally characterized as 6\u2032-O-phosphoryl-[O-\u03b2-d-galactosaminyl-(1\u2032\u21926)-d-glucose]. 4. C-substance contains free amino groups accessible to attack by 1-fluoro-2,4-dinitrobenzene and nitrous acid. 5. Choline phosphate and ribitol phosphate are units in the polymer. 6. Treatment with hot alkali gave a fragment comprising phosphate, d-galactosamine, d-glucose, diaminotrideoxyhexose and ribitol in the molecular proportions 2:1:1:1:1. 7. After selective N-acetylation, the fragment contained one of its phosphate groups as a phosphomonoester and one as a phosphodiester, shown by potentiometric titration and by treatment with a phosphomonoesterase. 8. C-substance from seven other strains of Pneumococcus possesses a structure common to that described for the strain 1\u2013192R. 9. Capsular materials from 26 different strains of Pneumococcus were analysed for suspected contamination by C-substance. In 19 cases the presence of C-substance with the normal structure was demonstrated, and in the remaining seven cases the contaminating C-substance was probably similarly constituted. 10. F-substance was isolated and the associated fatty acid material analysed.<\/jats:p>","DOI":"10.1042\/bj1100573","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:37:20Z","timestamp":1439501840000},"page":"573-582","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":140,"title":["Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate"],"prefix":"10.1042","volume":"110","author":[{"given":"D E","family":"Brundish","sequence":"first","affiliation":[{"name":"Microbiological Chemistry Research Laboratory, Department of Organic Chemistry, University of Newcastle upon Tyne, NE1 7RU"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Baddiley","sequence":"additional","affiliation":[{"name":"Microbiological Chemistry Research Laboratory, Department of Organic Chemistry, University of Newcastle upon Tyne, NE1 7RU"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/110\/3\/573\/762248\/bj1100573.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/110\/3\/573\/762248\/bj1100573.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T21:08:50Z","timestamp":1638220130000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/110\/3\/573\/16196\/Pneumococcal-C-substance-a-ribitol-teichoic-acid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,12,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1968,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1100573","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1968,12,1]]}}}