{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T14:52:17Z","timestamp":1648911137202},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1970,3,1]]},"abstract":"<jats:p>The destruction of 125I-labelled insulin by an enzyme system from rat adipose tissue was studied. The system was located in the particulate fraction. Activity was assayed by the amount of 125I-labelled degradation products rendered soluble in trichloroacetic acid. The system was heat-labile, with an alkaline pH optimum. The velocity of the reaction varied directly with the enzyme concentration. Paper chromatography of the degradation products showed six ninhydrin-sensitive areas with radioactivity coinciding with three of them. Albumin inhibited the system; ribonuclease did not. Although only 25% of the total 125I-label was detected by this assay, results with insulin-specific assays suggested that most (80\u201390%) of the hormone was inactivated. Possible interpretations of these results are discussed. The particulate fractions of other tissues were also studied.<\/jats:p>","DOI":"10.1042\/bj1160825","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:26:00Z","timestamp":1439234760000},"page":"825-831","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Degredation of insulin by a particulate fraction from adipose tissue"],"prefix":"10.1042","volume":"116","author":[{"given":"Kenneth","family":"Sumner","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Upstate Medical Center, Syracuse, N.Y. 13210, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Doisy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Upstate Medical Center, Syracuse, N.Y. 13210, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/116\/5\/825\/766568\/bj1160825.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/116\/5\/825\/766568\/bj1160825.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T18:48:22Z","timestamp":1637952502000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/116\/5\/825\/14767\/Degredation-of-insulin-by-a-particulate-fraction"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,3,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1970,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1160825","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1970,3,1]]}}}