{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T04:55:20Z","timestamp":1694926520662},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1970,5,1]]},"abstract":"<jats:p>1. The metabolism in vitro and microsomal interactions of (+)-amphetamine, (\u2212)-amphetamine, (+)-benzphetamine and (\u2212)-benzphetamine were studied with hepatic microsomes from phenobarbitone-pretreated male rabbits. 2. (+)-Benzphetamine was N-demethylated 30\u201335% faster than (\u2212)-benzphetamine, but the apparent Michaelis constants for the two enantiomers were similar. 3. (\u2212)-Amphetamine was deaminated about 200% faster than (+)-amphetamine. 4. The benzphetamine enantiomers gave qualitatively and quantitatively identical type I microsomal difference spectra (peak, 390nm; trough, 425nm) indicating identical apparent binding affinities for microsomes and identical spectral changes at maxima (\u0394Emax. values). 5. The amphetamine enantiomers gave qualitatively identical type II microsomal difference spectra (peak, 433nm; trough, 395nm). However, the type II spectral data indicated that (+)-amphetamine had a markedly higher apparent binding affinity than (\u2212)-amphetamine for microsomes. The amphetamine enantiomers gave identical \u0394Emax. values. 6. The benzphetamine enantiomers (0.5mm) enhanced the rate of microsomal cytochrome P-450 reduction by NADPH by 400\u2013500%, (+)-benzphetamine enhancing the rate 20\u201325% more than (\u2212)-benzphetamine. 7. The amphetamine enantiomers decreased the rate of microsomal cytochrome P-450 reduction by NADPH. At a concentration of 2mm, (+)-amphetamine decreased the rate more than (\u2212)-amphetamine. 7. All four enantiomers enhanced microsomal NADPH oxidation.<\/jats:p>","DOI":"10.1042\/bj1170833","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:27:27Z","timestamp":1439234847000},"page":"833-841","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["The metabolism <i>in vitro<\/i> and hepatic microsomal interactions of some enantiomeric drug substrates"],"prefix":"10.1042","volume":"117","author":[{"given":"David S.","family":"Hewick","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Iowa College of Medicine, Iowa City, Iowa 52240, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James R.","family":"Fouts","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Iowa College of Medicine, Iowa City, Iowa 52240, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/117\/5\/833\/767912\/bj1170833.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/117\/5\/833\/767912\/bj1170833.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T18:49:38Z","timestamp":1637952578000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/117\/5\/833\/14843\/The-metabolism-in-vitro-and-hepatic-microsomal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1970,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1170833","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1970,5,1]]}}}