{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:09:36Z","timestamp":1758280176336},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1970,6,1]]},"abstract":"<jats:p>1. After incubation of Escherichia coli with N-iodo[1,2-14C2]acetylglucosamine, 95\u201399% of the 14C taken up by whole cells is located in a cold-trichloroacetic acid-soluble fraction. Two major components of this fraction are S-carboxymethylcysteine and S-carboxymethylglutathione. The same compounds accumulate during incubation with iodo[14C]acetate but not with iodo[14C]acetamide. The amount of 14C associated with a cold-trichloroacetic acid-insoluble fraction are comparable for all three alkylating agents. After incubation with iodo[14C]acetamide, 50% of the label bound to whole cells is recoverable in a cold-trichloroacetic acid-insoluble fraction. 2. Uptake and incorporation of 14C from [U-14C]glycerol is blocked at an early stage by N-iodoacetylglucosamine. No specific inhibition of macromolecular synthesis could be demonstrated. 3. Mutants selected for resistance to iodoacetate are partially resistant to iodoacetate and N-iodoacetylglucosamine, but show no resistance to iodoacetamide. 4. Mutants selected for resistance to N-iodoacetylglucosamine are not resistant to iodoacetate or iodoacetamide, and are defective in their ability to grow on N-acetylglucosamine. Resistance to N-iodoacetylglucosamine is not absolute, and depends on the presence of glucose or certain other sugars; there is no resistance during growth on maltose, glycerol or succinate. 5. Absolute resistance can be achieved by selecting for a second mutation conferring resistance during growth on maltose; double mutants isolated by this procedure are unable to grow on N-acetylglucosamine and grow poorly on glucosamine. Resistant single mutants have a slightly diminished uptake of N-acetyl[1-14C]glucosamine, but in resistant double mutants the uptake of both [1-14C]glucosamine and N-acetyl[1-14C]glucosamine is severely diminished. 6. These observations are consistent with the presence of two permeases for N-acetylglucosamine, one that also permits uptake of glucosamine and another that allows entry of methyl 2-acetamido-2-deoxy-\u03b1-d-glucoside. N-Iodoacetylglucosamine can gain entry to the cell by both permeases.<\/jats:p>","DOI":"10.1042\/bj1180081","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:28:37Z","timestamp":1439234917000},"page":"81-87","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An examination of the inhibitory effects of <i>N<\/i>-iodoacetylglucosamine on <i>Escherichia coli<\/i> and isolation of resistant mutants"],"prefix":"10.1042","volume":"118","author":[{"given":"R. J.","family":"White","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. W.","family":"Kent","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/118\/1\/81\/767859\/bj1180081.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/118\/1\/81\/767859\/bj1180081.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T13:17:55Z","timestamp":1637932675000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/118\/1\/81\/4560\/An-examination-of-the-inhibitory-effects-of-N"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,6,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1970,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1180081","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1970,6,1]]}}}