{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:26:05Z","timestamp":1760646365349},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1972,6,1]]},"abstract":"<jats:p>1. Preincubation of the immature rat uterus under physiological conditions was found to increase its subsequent ability to transport \u03b1-aminoisobutyric acid, l-proline, l-alanine and 1-aminocyclopentanecarboxylic acid. Uptakes of l-valine, l-phenylalanine and l-leucine were not affected. With \u03b1-aminoisobutyric acid, a doubling of the uptake could be obtained after a 3\u20135h preincubation period. Uteri from oestradiol-primed rats gave increases similar to those found in tissues from untreated animals. In both cases the preincubation increased the Vmax. of \u03b1-aminoisobutyric acid uptake but did not affect the Km. 2. The conditions during the preincubation period determined the increase in subsequent uptake of \u03b1-aminoisobutyric acid. No increase in uptake was found if the preincubation was carried out at 1\u00b0C, in the presence of cyanide or dinitrophenol, under anaerobiosis or with a concentration of puromycin that depressed incorporation of l-leucine into protein by 95%. The puromycin was also shown to prevent the increase in Vmax. normally found after the preincubation period. In addition, no increase was found if Na+was omitted from the preincubation medium. Other inorganic ions had smaller effects. 3. The uptake of \u03b1-aminoisobutyric acid by uteri before and after a preincubation period showed the same general patterns of sensitivity to competitive inhibitors, K+, pH, temperature and 2,4-dinitrophenol. 4. The results suggest that the preincubation leads to an increase in a protein component of the \u2018A system\u2019 for amino acid transport in the uterus, and that metabolic energy is required for the reactions involved.<\/jats:p>","DOI":"10.1042\/bj1280019","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:44:32Z","timestamp":1439235872000},"page":"19-27","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Transport of amino acids into the oestrogen-primed uterus. Enhancement of the uptake by a preliminary incubation"],"prefix":"10.1042","volume":"128","author":[{"given":"Thomas R.","family":"Riggs","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry, University of Michigan, Ann Arbor, Mich. 48104, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary W.","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, University of Michigan, Ann Arbor, Mich. 48104, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/128\/1\/19\/777955\/bj1280019.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/128\/1\/19\/777955\/bj1280019.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T18:25:55Z","timestamp":1637951155000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/128\/1\/19\/12640\/Transport-of-amino-acids-into-the-oestrogen-primed"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,6,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1972,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1280019","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1972,6,1]]}}}