{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T14:07:26Z","timestamp":1648994846383},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1972,6,1]]},"abstract":"<jats:p>The equilibrium and kinetics of ethyl isocyanide binding to ferroperoxidase were studied. At pH9.1 the results of both studies are consistent with a single-process model with an affinity constant of 95m\u22121 and combination and dissociation constants of 2.2\u00d7103m\u22121\u00b7s\u22121 and 23s\u22121 respectively. Ethyl isocyanide is not bound significantly at pH values lower than 6.0, and in this behaviour and the pH-dependence of the affinity constant, similarities exist between isocyanide and cyanide binding. The enthalpy of the process measured by equilibrium methods is \u221259kJ\/mol (\u221214kcal\/mol). At pH values below 9, the ethyl isocyanide adduct changes in a slow time-dependent manner, giving rise to a new species. These changes are reversible on increasing the pH. The results are discussed in relation to other known information about ligand binding to ferroperoxidase and to myoglobin.<\/jats:p>","DOI":"10.1042\/bj1280377","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:44:32Z","timestamp":1439235872000},"page":"377-382","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The binding of ethyl isocyanide to ferroperoxidase"],"prefix":"10.1042","volume":"128","author":[{"given":"Charles","family":"Phelps","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K., and Centro di Biologia Moleculare, Istituto di Chimica Biologica, Citta Universitaria, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eraldo","family":"Antonini","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K., and Centro di Biologia Moleculare, Istituto di Chimica Biologica, Citta Universitaria, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Brunori","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K., and Centro di Biologia Moleculare, Istituto di Chimica Biologica, Citta Universitaria, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/128\/2\/377\/777275\/bj1280377.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/128\/2\/377\/777275\/bj1280377.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T14:31:07Z","timestamp":1637937067000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/128\/2\/377\/6625\/The-binding-of-ethyl-isocyanide-to-ferroperoxidase"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,6,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1972,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1280377","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1972,6,1]]}}}