{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T20:35:15Z","timestamp":1700771715868},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1972,8,1]]},"abstract":"<jats:p>The RNA of the blue\u2013green alga Anacystis nidulans contains three ribosomal RNA species with molecular weights of 0.56\u00d7106, 0.9\u00d7106, and 1.1\u00d7106if the RNA is extracted in the absence of Mg2+. The 0.9\u00d7106mol.wt. rRNA is extremely slowly labelled in32P-incorporation experiments. This rRNA may be a cleavage product of the 1.1\u00d7106mol.wt. rRNA from the ribosomes of cells in certain physiological states (e.g. light-deficiency during growth). The cleavage of the 1.1\u00d7106mol.wt. rRNA during the extraction procedure can be prevented by the addition of 10mm-MgCl2.32P-pulse-labelling studies demonstrate the rapid synthesis of two ribosomal precursor RNA species. One precursor RNA migrating slightly slower than the 1.1\u00d7106mol.wt. rRNA appears much less stable than the other precursor RNA, which shows the electrophoretic behaviour of the 0.7\u00d7106mol.wt. rRNA. Our observations support the close relationship between bacteria and blue\u2013green algae also with respect to rRNA maturation. The conversion of the ribosomal precursor RNA species into 0.56\u00d7106- and 1.1\u00d7106-mol.wt. rRNA species requires Mg2+in the incubation medium.<\/jats:p>","DOI":"10.1042\/bj1290135","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:46:11Z","timestamp":1439235971000},"page":"135-140","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in <i>Anacystis nidulans<\/i>"],"prefix":"10.1042","volume":"129","author":[{"given":"A.","family":"Szalay","sequence":"first","affiliation":[{"name":"Institute of Plant Biochemistry, German Academy of Sciences, 401 Halle (Saale), German Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Munsche","sequence":"additional","affiliation":[{"name":"Institute of Plant Biochemistry, German Academy of Sciences, 401 Halle (Saale), German Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Wollgiehn","sequence":"additional","affiliation":[{"name":"Institute of Plant Biochemistry, German Academy of Sciences, 401 Halle (Saale), German Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Parthier","sequence":"additional","affiliation":[{"name":"Institute of Plant Biochemistry, German Academy of Sciences, 401 Halle (Saale), German Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/129\/1\/135\/778186\/bj1290135.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/129\/1\/135\/778186\/bj1290135.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T14:44:28Z","timestamp":1637937868000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/129\/1\/135\/7016\/Ribosomal-ribonucleic-acid-and-ribosomal-precursor"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,8,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1972,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1290135","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1972,8,1]]}}}