{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T18:49:36Z","timestamp":1758394176372},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,1,1]]},"abstract":"<jats:p>Antisera were raised to a partially purified preparation of human liver hexosaminidase and to highly purified preparations of hexosaminidase isoenzymes A and B. All the antisera precipitated the enzyme in an enzymically active form, which could be located on immunodiffusion and immunoelectrophoretic gels by using a histochemical substrate. The antisera to the purified isoenzymes were shown to react with hexosaminidase from human liver, kidney, brain and spleen, but did not cross-react with human liver \u03b2-glucosidase, \u03b2-galactosidase, \u03b1-mannosidase, \u03b2-xylosidase, arylsulphatase or acid phosphatase. Hexosaminidases A and B were immunologically identical. The immunological properties of the hexosaminidases from livers of patients with three types of GM2-gangliosidoses were closely similar. No evidence could be found for cross-reacting material in enzyme-deficient states.<\/jats:p>","DOI":"10.1042\/bj1310091","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:48:26Z","timestamp":1439236106000},"page":"91-96","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Immunological properties of <i>N<\/i>-acetyl-\u03b2-<scp>d<\/scp>-glucosaminidase of normal human liver and of GM2-gangliosidosis liver"],"prefix":"10.1042","volume":"131","author":[{"given":"M.","family":"Carroll","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Queen Elizabeth College, Campden Hill, London W.8, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Queen Elizabeth College, Campden Hill, London W.8, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/131\/1\/91\/560508\/bj1310091.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/131\/1\/91\/560508\/bj1310091.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T14:58:37Z","timestamp":1637938717000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/131\/1\/91\/7566\/Immunological-properties-of-N-acetyl-d"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1973,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1310091","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1973,1,1]]}}}