{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:30:13Z","timestamp":1790843413567,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,3,1]]},"abstract":"<jats:p>Ethanol oxidation by rat liver catalase (the \u2018peroxidatic\u2019 reaction) was studied quantitatively with respect to the rate of H2O2 generation, catalase haem concentration, ethanol concentration and the steady-state concentration of the catalase\u2013H2O2 intermediate (Compound I). At a low ratio of H2O2-generation rate to catalase haem concentration, the rate of ethanol oxidation was independent of the catalase haem concentration. The magnitude of the inhibition of ethanol oxidation by cyanide was not paralleled by the formation of the catalase\u2013cyanide complex and was altered greatly by varying either the ethanol concentration or the ratio of the rate of H2O2 generation to catalase haem concentration. The ethanol concentration producing a half-maximal activity was also dependent on the ratio of the H2O2-generation rate to catalase haem concentration. These phenomena are explained by changes in the proportion of the \u2018catalatic\u2019 and \u2018peroxidatic\u2019 reactions in the overall H2O2-decomposition reaction. There was a correlation between the proportion of the \u2018peroxidatic\u2019 reaction in the overall catalase reaction and the steady-state concentration of the catalase\u2013H2O2 intermediate. Regardless of the concentration of ethanol and the rate of H2O2 generation, a half-saturation of the steady state of the catalase\u2013H2O2 intermediate indicated that about 45% of the H2O2 was being utilized by the ethanol-oxidation reaction. The results reported show that the experimental results in the study on the \u2018microsomal ethanol-oxidation system\u2019 may be reinterpreted and the catalase \u2018peroxidatic\u2019 reaction provides a quantitative explanation for the activity hitherto attributed to the \u2018microsomal ethanol-oxidation system\u2019.<\/jats:p>","DOI":"10.1042\/bj1310555","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:48:26Z","timestamp":1439236106000},"page":"555-563","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":173,"title":["The characteristics of the \u2018peroxidatic\u2019 reaction of catalase in ethanol oxidation"],"prefix":"10.1042","volume":"131","author":[{"given":"Nozomu","family":"Oshino","sequence":"first","affiliation":[{"name":"Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, Pa. 19174, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiko","family":"Oshino","sequence":"additional","affiliation":[{"name":"Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, Pa. 19174, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britton","family":"Chance","sequence":"additional","affiliation":[{"name":"Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, Pa. 19174, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/131\/3\/555\/560863\/bj1310555.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/131\/3\/555\/560863\/bj1310555.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T15:04:03Z","timestamp":1637939043000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/131\/3\/555\/7714\/The-characteristics-of-the-peroxidatic-reaction-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1973,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1310555","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1973,3,1]]}}}