{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T18:04:35Z","timestamp":1698775475588},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,7,15]]},"abstract":"<jats:p>A method is described for the preparation of mitochondria from the slime mould Physarum polycephalum; the mitochondria were not coupled. P. polycephalum mitochondria oxidized added NADH via a rotenone-insensitive pathway, but the oxidation of malate plus glutamate was rotenone sensitive; both of these substrates reduced much less cytochrome b than did succinate, in both aerobic and anaerobic steady states. Spectroscopy at 77\u00b0K separated three absorption maxima in the \u03b1-band region, at 560nm, 553nm and one at 547nm due to cytochrome c. The absorption at 553nm was increased in the aerobic steady state by the addition of 2-heptyl-4-hydroxyquinoline N-oxide, suggesting that it was due to a b-type cytochrome. All three absorption maxima appeared in the aerobic steady state after the addition of a range of substrates. The respiratory activity with different substrates and the response to inhibitors of respiration were similar to those previously described for fungus mitochondria (Weiss et al., 1970; Erickson &amp; Ashworth, 1969). When grown under conditions of haem limitation the mitochondria contained a lower concentration of cytochromes than normal.<\/jats:p>","DOI":"10.1042\/bj1340745","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:50:06Z","timestamp":1439236206000},"page":"745-751","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The electron-transport system of mitochondria from the slime mould <i>Physarum polycephalum<\/i>"],"prefix":"10.1042","volume":"134","author":[{"given":"Roger","family":"Barnes","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emer M.","family":"Colleran","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. T. G.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/134\/3\/745\/780605\/bj1340745.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/134\/3\/745\/780605\/bj1340745.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T15:09:41Z","timestamp":1637939381000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/134\/3\/745\/7846\/The-electron-transport-system-of-mitochondria-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,7,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1973,7,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1340745","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1973,7,15]]}}}