{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:36:44Z","timestamp":1761763004452},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,12,1]]},"abstract":"<jats:p>1. A preparation of a partly purified mitochondrial outer-membrane fraction suitable for kinetic investigations of monoamine oxidase is described. 2. An apparatus suitable for varying the O2 concentration in a spectrophotometer cuvette is described. 3. The reaction catalysed by the membrane-bound enzyme is shown to proceed by a double-displacement (Ping Pong) mechanism, and a formal mechanism is proposed. 4. KCN, NaN3, benzyl cyanide and 4-cyanophenol are shown to be reversible inhibitors of the enzyme. 5. The non-linear reciprocal plot obtained with impure preparations of benzylamine, which is typical of high substrate inhibition, is shown to be due to aldehyde contamination of the substrate.<\/jats:p>","DOI":"10.1042\/bj1350735","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:50:41Z","timestamp":1439236241000},"page":"735-750","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":88,"title":["The reaction pathway of membrane-bound rat liver mitochondrial monoamine oxidase"],"prefix":"10.1042","volume":"135","author":[{"given":"Miles D.","family":"Houslay","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith F.","family":"Tipton","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/135\/4\/735\/561882\/bj1350735.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/135\/4\/735\/561882\/bj1350735.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T16:09:55Z","timestamp":1637942995000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/135\/4\/735\/9254\/The-reaction-pathway-of-membrane-bound-rat-liver"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,12,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1973,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1350735","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1973,12,1]]}}}