{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T21:15:18Z","timestamp":1699650918421},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1974,8,15]]},"abstract":"<jats:p>The release of growth hormone from heifer anterior pituitary slices and the cyclic AMP content of the slices were increased by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, both increases being related to inhibitor concentration over the range 0.1\u20131.0mm. Neither Ba2+(6.9 or 2.3mm), K+(72mm), nor p-chloromercuribenzoate (20\u03bcm) had any effect on pituitary cyclic AMP content over a 20min period. 3-Isobutyl-1-methylxanthine potentiated the release of growth hormone in response to Ba2+(2.3mm) and K+(24mm), but the degree of potentiation did not depend on inhibitor concentration in the same way as did tissue cyclic AMP content. 3-Isobutyl-1-methylxanthine decreased the concentration of K+required to give maximum stimulation of growth-hormone release, but did not significantly increase the maximum response to Ba2+. Growth-hormone release in the presence of prostaglandin E2 (1\u03bcm) was increased by 3-isobutyl-1-methylxanthine and was inhibited by the prostaglandin antagonist, 7-oxa-13-prostynoic acid, although this antagonist increased the pituitary cyclic AMP concentration and potentiated the prostaglandin E2-induced rise in cyclic AMP content. The stimulation of growth-hormone release by p-chloromercuribenzoate was not potentiated by 3-isobutyl-1-methylxanthine. The data suggest that Ba+and K+act at the same point in the secretory process as 3-isobutyl-1-methylxanthine, although by a different mechanism, and that p-chloromercuribenzoate has a different point of action.<\/jats:p>","DOI":"10.1042\/bj1420295","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:54:43Z","timestamp":1439236483000},"page":"295-300","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Increase in pituitary adenosine 3\u2032:5\u2032-cyclic monophosphate content and potentiation of growth-hormone release from heifer anterior pituitary slices incubated in the presence of 3-isobutyl-1-methylxanthine"],"prefix":"10.1042","volume":"142","author":[{"given":"J. George","family":"Schofield","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"McPherson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/142\/2\/295\/781134\/bj1420295.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/142\/2\/295\/781134\/bj1420295.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T16:29:45Z","timestamp":1637944185000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/142\/2\/295\/9683\/Increase-in-pituitary-adenosine-3-5-cyclic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,8,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1974,8,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1420295","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1974,8,15]]}}}