{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:57:28Z","timestamp":1758279448812},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1974,12,1]]},"abstract":"<jats:p>1. 30S and 50S ribosomal subunits of Escherichia coli were treated with N-[2,3-14C]-ethylmaleimide and iodo[14C]acetamide. 2. The proteins in the native subunits which reacted with the reagents were S1,\u2021 S2, S12, S13, S18, S21, L2, L5, L6, L10, L11, L15, L17, L20, L26+28 and L27. 3. Several proteins, such as S1, S12, S14, S18, L2, L6, L10, L11 and either L26 or 28, had thiol groups in an oxidized form and reacted to a greater extent after reduction with \u03b2-mercaptoethanol or dithiothreitol. 4. The total number of thiol groups in 30S and 50S subunits was determined as 16\u201317 and 26\u201327 respectively. The total number of thiol groups in each ribosomal protein was also determined. 5. The reaction of 30S and 50S subunits with iodoacetamide under several different conditions established the order of reactivity of thiol groups.<\/jats:p>","DOI":"10.1042\/bj1430599","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:55:25Z","timestamp":1439236525000},"page":"599-606","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Topography of <i>Escherichia coli<\/i> ribosomal proteins. The order of reactivity of thiol groups"],"prefix":"10.1042","volume":"143","author":[{"given":"Anastasia","family":"Bakardjieva","sequence":"first","affiliation":[{"name":"Max Planck Institut f\u00fcr Molekulare Genetik, Abteliung Wittmann, 1 Berlin-Dahlem, Ihnestr. 63, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert R.","family":"Crichton","sequence":"additional","affiliation":[{"name":"Max Planck Institut f\u00fcr Molekulare Genetik, Abteliung Wittmann, 1 Berlin-Dahlem, Ihnestr. 63, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/143\/3\/599\/563328\/bj1430599.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/143\/3\/599\/563328\/bj1430599.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T16:48:16Z","timestamp":1637945296000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/143\/3\/599\/9855\/Topography-of-Escherichia-coli-ribosomal-proteins"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,12,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1974,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1430599","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1974,12,1]]}}}