{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T14:06:53Z","timestamp":1767276413348},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,12,1]]},"abstract":"<jats:p>1. The choline dehydrogenase (EC 1.1.99.1) WAS SOLUBILIZED FROM ACETONE-DRIED POWDERS OF RAT LIVER MITOCHONDRIA BY TREATMENT WITH Naja naja venom. 2. The kinetics of the reaction of enzyme with phenazine methosulphate and ubiquinone-2 as electron acceptors were investigated. 3. With both electron acceptors the reaction mechanism appears to involve a free, modified-enzyme intermediate. 4. With some electron acceptors the maximum velocity of the reaction is independent of the nature of the acceptor. With phenazine methosulphate and ubiquinone-2 as acceptors the Km value for choline is also independent of the nature of the acceptor molecule. 5. The mechanism of the Triton X-100-solubilized enzyme is apparently the smae as that for the snake venom solubilized enzyme.<\/jats:p>","DOI":"10.1042\/bj1510677","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:00:10Z","timestamp":1439236810000},"page":"677-683","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The reaction of choline dehydrogenase with some electron acceptors"],"prefix":"10.1042","volume":"151","author":[{"given":"M C","family":"Barrett","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A P","family":"Dawson","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/151\/3\/677\/566517\/bj1510677.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/151\/3\/677\/566517\/bj1510677.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T16:54:33Z","timestamp":1637945673000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/151\/3\/677\/10010\/The-reaction-of-choline-dehydrogenase-with-some"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,12,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1975,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1510677","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1975,12,1]]}}}