{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T05:31:12Z","timestamp":1770442272860,"version":"3.49.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,12,1]]},"abstract":"<jats:p>A combined mitochondrial and light mitochondrial fraction and a microsomal fraction were isolated from bovine thyroid gland and fractionated further in a B-XIV zonal rotor. A density gradient ranging from 20 to 50% (w\/w) sucrose was used. The rotor was operated for 3 h at 45 000 rev.\/min. All manipulations were performed at 4 degrees C and at pH 7.4. 2. Membranous material was recovered in two zones: zone I, containing microsomal material derived from both smooth endoplasmic reticulum and plasma membranes and probably also from other smooth membranes; zone II, containing material from rough endoplasmic reticulum. 3. Increasing the pH of the medium up to 8.6, or the addition of Mg2+ to the medium resulted in the formation of a single zone at intermediate densities (aggregation of membranes?). An analogous effect was obtained after treatment with Pb (NO3) 2. 4. In the presence of heparin (50 i.u.\/ml) the bulk of the membranes was found in zone I. This was due to the release of ribosomes from the rough endoplasmic reticulum.<\/jats:p>","DOI":"10.1042\/bj1520601","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:01:08Z","timestamp":1439236868000},"page":"601-607","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Subcellular structure of bovine thyroid gland. A study on bovine thyroid membranes by buoyant-density-gradient centrifugation in a B-XIV zonal rotor"],"prefix":"10.1042","volume":"152","author":[{"given":"H J","family":"Hilderson","sequence":"first","affiliation":[{"name":"RUCA-Laboratory for Human Biochemistry, University of Antwerp, Groenenborgerlaan 171, B2020 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M J","family":"De Wolf","sequence":"additional","affiliation":[{"name":"RUCA-Laboratory for Human Biochemistry, University of Antwerp, Groenenborgerlaan 171, B2020 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A R","family":"Lagrou","sequence":"additional","affiliation":[{"name":"RUCA-Laboratory for Human Biochemistry, University of Antwerp, Groenenborgerlaan 171, B2020 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W S","family":"Dierick","sequence":"additional","affiliation":[{"name":"RUCA-Laboratory for Human Biochemistry, University of Antwerp, Groenenborgerlaan 171, B2020 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/152\/3\/601\/566156\/bj1520601.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/152\/3\/601\/566156\/bj1520601.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T17:08:51Z","timestamp":1637946531000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/152\/3\/601\/10481\/Subcellular-structure-of-bovine-thyroid-gland-A"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,12,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1975,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1520601","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1975,12,1]]}}}