{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:50:47Z","timestamp":1760889047514},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1976,11,15]]},"abstract":"<jats:p>The hepatic synthesis and accumulation of S-adenosylhomocysteine, S-adenosylmethionine and polyamines were studied in normal and vitamin B-6-deficient male albino rats. A method involving a single chromatography on a phosphocellulose column was developed for the determination of S-adenosylhomocysteine and S-adenosylmethionine from tissue samples. Feeding the rat with pyridoxine-deficient diet for 3 or 6 weeks resulted in a four- to five-fold increase in the concentration of S-adenosylhomocysteine, whereas that of S-adenosylmethionine was only slighly elevated. The concentration of putrescine was decreased to half, that of spermidine was somewhat decreased and that of spermine remained fairly constant. The activities of L-ornithine decarboxylase, S-adenosyl-L-methionine decarboxylase, L-methionine adenosyltransferase and S-adenosyl-L-homocysteine hydrolase were moderately increased. S-Adenosylmethionine decarboxylase showed no requirement for pyridoxal 5\u2032-phosphate. The major effect of pyridoxine deficiency of S-adenosylmethionine metabolism seems to be a block in the utilization of S-adenosylhomocysteine, resulting in the accumulation of this metabolite to a concentration that may inhibit biological methylation reactions.<\/jats:p>","DOI":"10.1042\/bj1600287","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:04:42Z","timestamp":1439237082000},"page":"287-294","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["A new method for the assay of tissue. <i>S<\/i>-adenosylhomocysteine and <i>S<\/i>-adenosylmethione. Effect of pyridoxine deficiency on the metabolism of <i>S<\/i>-adenosylhomocysteine, <i>S<\/i>-adenosylmethionine and polyamines in rat liver"],"prefix":"10.1042","volume":"160","author":[{"given":"T O","family":"Eloranta","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Kuopio, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E O","family":"Kajander","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Kuopio, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A M","family":"Raina","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Kuopio, Kuopio, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/160\/2\/287\/782079\/bj1600287.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/160\/2\/287\/782079\/bj1600287.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T17:34:15Z","timestamp":1637948055000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/160\/2\/287\/11233\/A-new-method-for-the-assay-of-tissue-S"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,11,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1976,11,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1600287","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1976,11,15]]}}}