{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T05:39:26Z","timestamp":1704087566942},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1977,9,15]]},"abstract":"<jats:p>X-band electron-paramagnetic-resonance spectroscopy at 4.2\u201377K combined with measurements of oxidation-reduction potential was used to identify iron\u2013sulphur centres in Arum maculatum (cuckoo-pint) mitochondria. In the oxidized state a signal with a derivative maximum at g = 2.02 was assigned to succinate dehydrogenase centre S-3. Unreduced particles showed additional signals at g = 2.04 and 1.98 (at 9.2 GHz), which may be due to a spin-spin interaction. In the reduced state a prominent signal at g = 1.93 and 2.02 was resolved into at least three components that could be assigned to centres S-1 and S-2 of succinate dehydrogenase (midpoint potentials \u22127 and \u2212240 mV respectively at pH 7.2) and a small amount of centre N-1b (e'o= \u2212240 mV) of NADH-ubiquinone reductase. In addition, changes in line shape around \u221210 mV indicated the presence of a fourth component in this signal. The latter was more readily reduced by NADH than by succinate, suggesting that it might be associated with the external NADH dehydrogenase. The iron-sulphur centres of NADH-ubiquinone reductase were present in an unusually low concentration, indicating that the alternative, non-phosphorylating, NADH dehydrogenase containing a low number of iron-sulphur centres may be responsible for most of the high rate of oxidation of NADH.<\/jats:p>","DOI":"10.1042\/bj1660347","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:08:11Z","timestamp":1439237291000},"page":"347-355","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Iron-sulphur centres in mitochondria from <i>Arum maculatum<\/i> spadix with very high rates of cyanide-resistant respiration"],"prefix":"10.1042","volume":"166","author":[{"given":"R","family":"Cammack","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J M","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/166\/3\/347\/569538\/bj1660347.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/166\/3\/347\/569538\/bj1660347.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T17:51:26Z","timestamp":1637949086000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/166\/3\/347\/11676\/Iron-sulphur-centres-in-mitochondria-from-Arum"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,9,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1977,9,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1660347","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1977,9,15]]}}}