{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:10:05Z","timestamp":1758280205294},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1978,2,15]]},"abstract":"<jats:p>Control of the rate of cardiac cell division by oxygen occurs most probably by altering the redox state of a control substance, e.g. NAD+\u21ccNADH. NAD+(and not NADH) forms poly(ADP-ribose), an inhibitor of DNA synthesis, in a reaction catalysed by poly(ADP-ribose) polymerase. Lower partial pressure of oxygen, which increases the rate of division, would shift NAD+\u2192NADH, decrease poly(ADP-ribose) synthesis, and increase DNA synthesis. Chick-embryo heart cells grown in culture in 20% O2 (in which they divide more slowly than in 5% O2) did exhibit greater poly(ADP-ribose) polymerase activity (+83%, P&amp;lt;0.001) than when grown in 5% O2. Reaction product was identified as poly(ADP-ribose) by its insensitivity to deoxyribonuclease, ribonuclease, NAD glycohydrolase, Pronase, trypsin and micrococcal nuclease, and by its complete digestion with snake-venom phosphodiesterase to phosphoribosyl-AMP and AMP. Isolation of these digestion products by Dowex 1 (formate form) column chromatography and paper chromatography allowed calculation of average poly(ADP-ribose) chain length, which was 15\u201326% greater in 20% than in 5% O2. Thus in 20% O2 the increase in poly(ADP-ribose) formation results from chain elongation. Formation of new chains also occurs, probably to an even greater degree than chain elongation. Additionally, poly(ADP-ribose) polymerase has very different Km and Vmax. values and pH optima in 20% and 5% O2. These data suggest that poly(ADP-ribose) metabolism participates in the regulation of heart-cell division by O2, probably by several different mechanisms.<\/jats:p>","DOI":"10.1042\/bj1700387","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:10:16Z","timestamp":1439237416000},"page":"387-394","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Poly(adenosine diphosphate ribose) metabolism and regulation of myocardial cell growth by oxygen"],"prefix":"10.1042","volume":"170","author":[{"given":"Q. Perveen","family":"Ghani","sequence":"first","affiliation":[{"name":"Cardiovascular Research Institute and Departments of Medicine, Veterans Administration Hospital and University of California, San Francisco, CA 94143, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milton","family":"Hollenberg","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and Departments of Medicine, Veterans Administration Hospital and University of California, San Francisco, CA 94143, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/170\/2\/387\/782589\/bj1700387.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/170\/2\/387\/782589\/bj1700387.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T17:46:45Z","timestamp":1637948805000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/170\/2\/387\/11526\/Poly-adenosine-diphosphate-ribose-metabolism-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,2,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1978,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1700387","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1978,2,15]]}}}