{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:27:37Z","timestamp":1790476057252,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1979,1,1]]},"abstract":"<jats:p>Proteoglycan fractions were prepared from pig laryngeal cartilage. The effect of link-protein on the properties of proteoglycan-hyaluronate aggregates was examined by viscometry and analytical ultracentrifugation. Aggregates containing link-protein were more stable than link-free aggregates at neutral pH, at temperatures up to 50 degrees C and in urea (up to 4.0M). Oligosaccharides of hyaluronate were able to displace proteoglycans from link-free aggregates, but not from the link-stabilized aggregates. Both types of aggregate were observed in the ultracentrifuge, but at the concentration investigated (less than 2 mg\/ml) the link-free form was partially dissociated and the proportion aggregated varied with the pH and temperature and required more hyaluronate for saturation than did link-stabilized aggregate. The results showed that link-protein greatly strengthened the binding of proteoglycans to hyaluronate and suggest that under physiological conditions it \u2018locks\u2019 proteoglycans on to the hyaluronate chain.<\/jats:p>","DOI":"10.1042\/bj1770237","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T16:14:35Z","timestamp":1439223275000},"page":"237-247","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":286,"title":["The role of link-protein in the structure of cartilage proteoglycan aggregates"],"prefix":"10.1042","volume":"177","author":[{"given":"T E","family":"Hardingham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/177\/1\/237\/571651\/bj1770237.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/177\/1\/237\/571651\/bj1770237.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T13:01:52Z","timestamp":1637931712000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/177\/1\/237\/12094\/The-role-of-link-protein-in-the-structure-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1979,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1770237","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1979,1,1]]}}}