{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:38:57Z","timestamp":1759963137340},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1980,4,15]]},"abstract":"<jats:p>An intraperitoneal dose of CS2 (500mg\/kg) to male rats resulted in loss of liver microsomal mixed-function-oxidase activity (85% loss of biphenyl 4-hydroxylase), followed by denaturation of liver cytochrome P-450 to cytochrome P-420, and degradative loss of both cytochromes (50% loss). Losses of NADPH\u2013cytochrome c reductase (20%) and cytochrome b5 were considerably less. Intraperitoneal administration of CS2 (100mg\/kg) to rats pretreated wtih phenobarbitone or 3-methylcholanthrene resulted in similar losses, but the rate of destruction was greater with cytochrome P-450 than with cytochrome P-448. At 12h after intraperitoneal injection of CS2 to non-pretreated rats, a new cytochrome (P-448) appeared. Rat liver microsomal preparations incubated with CS2 in the presence of NADPH and O2 resulted in loss of cytochrome P-450 and mixed-function-oxidase activity directly related to the concentration of CS2 (10\u2013100\u03bcm) and to the period of incubation. Addition of EDTA (1mm) completely inhibited this destruction of cytochrome P-450 by CS2in vitro. Addition of CS2 to liver microsomal preparations resulted in moderate increases in the Ks values for type-I or type-II substrates, but these were insufficient to account for the inhibition of the mixed-function oxidases. We therefore suggest that desulphuration of CS2 leads to binding of the S to cytochrome P-450, denaturation of cytochrome P-450 to cytochrome P-420, and ultimately to destruction of these cytochromes by autoxidation.<\/jats:p>","DOI":"10.1042\/bj1880107","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:22:37Z","timestamp":1439238157000},"page":"107-112","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The effects of carbon disulphide on rat liver microsomal mixed-function oxidases, <i>in vivo<\/i> and <i>in vitro<\/i>"],"prefix":"10.1042","volume":"188","author":[{"given":"Maria J.","family":"Obrebska","sequence":"first","affiliation":[{"name":"Institute of Occupational Medicine, Lodz, Poland, and Department of Biochemistry, University of Surrey, Guildford, Surrey GU2 5XH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Kentish","sequence":"additional","affiliation":[{"name":"Institute of Occupational Medicine, Lodz, Poland, and Department of Biochemistry, University of Surrey, Guildford, Surrey GU2 5XH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis V.","family":"Parke","sequence":"additional","affiliation":[{"name":"Institute of Occupational Medicine, Lodz, Poland, and Department of Biochemistry, University of Surrey, Guildford, Surrey GU2 5XH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/188\/1\/107\/574554\/bj1880107.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/188\/1\/107\/574554\/bj1880107.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T13:58:03Z","timestamp":1637935083000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/188\/1\/107\/5557\/The-effects-of-carbon-disulphide-on-rat-liver"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,4,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1980,4,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1880107","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1980,4,15]]}}}