{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:42:18Z","timestamp":1762101738629},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1980,5,15]]},"abstract":"<jats:p>We have studied effects of single doses of 2-allyl-2-isopropylacetamide and ferric citrate on hepatic haem turnover in rats. Haem was pre-labelled by intraperitoneal administration of 5-amino-[4-14C]laevulinate 4h before other treatments. Computer-assisted analysis of the haem decay curve showed that at least two exponential components were involved implying two haem pools. In control rats the size of the rapidly-turning-over pool was 38% of the total. Treatment with 2-allyl-2-isopropylacetamide alone resulted in a 2.3-fold increase in the fractional size of this pool. Treatment with ferric citrate alone increased the size of this pool 1.8-fold; treatment with both agents together had no measurable effect beyond that produced by 2-allyl-2-isopropylacetamide. The apparent rate constant for disappearance of labelled haem from the first pool was not affected by treatment with 2-allyl-2-isopropylacetamide or ferric citrate, indicating that these treatments affect hepatic haem turnover primarily by altering the distribution of haem synthesized in the liver. The increased haem degradation after treatment with 2-allyl-2-isopropylacetamide alone was associated with the accumulation of \u2018green pigments\u2019 in the liver. These pigments were detectable 1.5h after drug treatment; their total amount increased steadily for 28h and then declined. Despite this increase in amount, radioactivity of the \u2018green pigment\u2019 fraction fell rapidly in a biphasic fashion. Some of the radioactivity that initially was found in the \u2018green pigment\u2019 fraction was later found in an aqueous fraction, not extractable by acidic ethyl acetate. Rats given ferric citrate together with 2-allyl-2-isopropylacetamide accumulated less \u2018green pigments\u2019.<\/jats:p>","DOI":"10.1042\/bj1880289","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:22:37Z","timestamp":1439238157000},"page":"289-295","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Metabolism of hepatic haem and \u2018green pigments\u2019 in rats given 2-allyl-2-isopropylacetamide and ferric citrate. A new model for hepatic haem turnover"],"prefix":"10.1042","volume":"188","author":[{"given":"Herbert L.","family":"Bonkowsky","sequence":"first","affiliation":[{"name":"Hepatology and Metabolism Research Laboratory and Departments of Medicine and Biochemistry, VA Medical Centre, White River Junction, VT 05001, U.S.A. and Dartmouth Medical School, Hanover, NH 03755, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Healey","sequence":"additional","affiliation":[{"name":"Hepatology and Metabolism Research Laboratory and Departments of Medicine and Biochemistry, VA Medical Centre, White River Junction, VT 05001, U.S.A. and Dartmouth Medical School, Hanover, NH 03755, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter R.","family":"Sinclair","sequence":"additional","affiliation":[{"name":"Hepatology and Metabolism Research Laboratory and Departments of Medicine and Biochemistry, VA Medical Centre, White River Junction, VT 05001, U.S.A. and Dartmouth Medical School, Hanover, NH 03755, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yash P.","family":"Mayer","sequence":"additional","affiliation":[{"name":"Department of Chemistry, State University of New York, Albany, NY 12222, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond","family":"Erny","sequence":"additional","affiliation":[{"name":"Hepatology and Metabolism Research Laboratory and Departments of Medicine and Biochemistry, VA Medical Centre, White River Junction, VT 05001, U.S.A. and Dartmouth Medical School, Hanover, NH 03755, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/188\/2\/289\/783442\/bj1880289.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/188\/2\/289\/783442\/bj1880289.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T17:24:33Z","timestamp":1637947473000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/188\/2\/289\/11007\/Metabolism-of-hepatic-haem-and-green-pigments-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,5,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,5,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1880289","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1980,5,15]]}}}