{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:51:10Z","timestamp":1758279070371},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1980,8,1]]},"abstract":"<jats:p>Cell extracts of myxamoebae of Physarum polycephalum have been prepared in such a way that they do not inhibit assembly of brain microtubule protein in vitro even at high extract-protein concentration. Co-polymers of these extracts and brain tubulin have been purified to constant stoichiometry and amoebal components identified by radiolabelling. Amoebal tubulin has been identified as having an alpha-subunit, mol.wt. 54 000, which co-migrates with brain alpha-tubulin and a beta-subunit, mol.wt. 50 000, which co-migrates with Tetrahymena ciliary beta-tubulin. Non-tubulin amoebal proteins that co-purify with tubulin during co-polymer formation have been shown to be essential for microtubule formation in the absence of glycerol and appear to be rather more effective than brain microtubule-associated proteins in stimulating assembly. The mitotic inhibitor griseofulvin (7-chloro-2\u2032,4,6-trimethoxy-6\u2032-methylspiro[benzofuran-2(3H),1\u2032-cyclohex-2\u2032-ene] \u22123,4\u2032-dione), which binds to brain microtubule-associated proteins and inhibits brain microtubule assembly in vitro, affected co-polymer microtubule protein in a similar way, but to a slightly greater extent.<\/jats:p>","DOI":"10.1042\/bj1890305","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:22:52Z","timestamp":1439238172000},"page":"305-312","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Identification and characterization of microtubule proteins from myxamoebae of <i>Physarum polycephalum<\/i>"],"prefix":"10.1042","volume":"189","author":[{"given":"A","family":"Roobol","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C I","family":"Pogson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Gull","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/189\/2\/305\/573959\/bj1890305.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/189\/2\/305\/573959\/bj1890305.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T14:19:48Z","timestamp":1637936388000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/189\/2\/305\/6132\/Identification-and-characterization-of-microtubule"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1890305","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1980,8,1]]}}}