{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:28:35Z","timestamp":1759332515908},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1980,8,15]]},"abstract":"<jats:p>Several measures of energy conservation, namely ADP\/O ratio, P\/O ratio, ATP\/O ratio and phosphorylation detected by continuous assay with purified firefly luciferase and luciferin, all show phosphorylation can occur with mung-bean mitochondria at cyanide concentrations sufficient to inhibit the cytochrome oxidase system. Phosphorylation in the presence of cyanide is uncoupler- oligomycin- and salicylhydroxamate-sensitive. The participation of phosphorylation site 1 is excluded, phosphorylation being attributable to a single phosphorylation site associated with the cyanide-insensitive oxidase. The cyanide-insensitive oxidase has also been shown to support a variety of other energy-linked functions, namely, Ca2+ uptake, reversed electron transport and the maintenance of a membrane potential detected by the dye probes 8-anilinonaphthalene-1-sulphonate and safranine. High concentrations of cyanide have uncoupler-like activity, decreasing the ADP\/O ratio and the t 1\/2 for the decay of a pH pulse through the the mitochondrial membrane. This uncoupler-like effect is most marked with aged mitochondria. The observations of energy conservation attributable to the cyanide-insensitive oxidase are compared with other reports where it is concluded that the alternative oxidase is uncoupled.<\/jats:p>","DOI":"10.1042\/bj1900349","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:23:49Z","timestamp":1439238229000},"page":"349-360","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Energy conservation by the plant mitochondrial cyanide-insensitive oxidase. Some additional evidence"],"prefix":"10.1042","volume":"190","author":[{"given":"S B","family":"Wilson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/190\/2\/349\/783761\/bj1900349.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/190\/2\/349\/783761\/bj1900349.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T12:17:25Z","timestamp":1637842645000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/190\/2\/349\/11863\/Energy-conservation-by-the-plant-mitochondrial"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,8,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,8,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1900349","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1980,8,15]]}}}