{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:26:04Z","timestamp":1760646364067},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1981,3,1]]},"abstract":"<jats:p>Mono[3H]propionyl-alpha-bungarotoxin, prepared with N-succinimidyl [2,3-3H]propionate (sp. radioactivity 50 Ci\/mmol) and purified to homogeneity by electrofocusing, retains its biological activity and stability. Rate constants for its binding to acetylcholine receptor were 4.4-fold lower than for unlabelled toxin; no dissociation was detectable. Analysis of enzymic digests of toxin showed 3H is located mainly of entirely in epsilon-propionyl-lysine.<\/jats:p>","DOI":"10.1042\/bj1930919","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:26:12Z","timestamp":1439238372000},"page":"919-923","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Tritiation of \u03b1-bungarotoxin with <i>N<\/i>-succinimidyl [2,3-3H]propionate. A useful reagent for labelling proteins"],"prefix":"10.1042","volume":"193","author":[{"given":"J O","family":"Dolly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E A V","family":"Nockles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M M S","family":"Lo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E A","family":"Barnard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/193\/3\/919\/564597\/bj1930919.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/193\/3\/919\/564597\/bj1930919.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T14:17:49Z","timestamp":1637849869000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/193\/3\/919\/17896\/Tritiation-of-bungarotoxin-with-N-succinimidyl-2-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1981,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1930919","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1981,3,1]]}}}