{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T17:18:40Z","timestamp":1785863920809,"version":"3.56.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1981,11,1]]},"abstract":"<jats:p>Adenosine 5\u2032-(S)-[16O,17O,18O]phosphate was pyrophosphorylated by the combined action of adenylate kinase and pyruvate kinase. The isotopomers of adenosine 5\u2032-[alpha-16O,17O,18O]triphosphate were hydrolysed by venom 5\u2032-nucleotide phosphodiesterase (Crotalus adamanteus) in H2(17)O. Analysis by 31P nuclear magnetic resonance spectroscopy of the resulting adenosine 5\u2032-[16O,17O,18O]phosphate, after cyclization and esterification, showed that the hydrolysis occurs with retention of configuration at phosphorus. The most likely explanation of this observation is that the enzymic hydrolysis involves a double displacement at phosphorus with a covalent nucleotidyl--enzyme intermediate on the reaction pathway.<\/jats:p>","DOI":"10.1042\/bj1990447","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:30:03Z","timestamp":1439238603000},"page":"447-451","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The stereochemical course of hydrolysis catalysed by snake venom 5\u2032-nucleotide phosphodiesterase"],"prefix":"10.1042","volume":"199","author":[{"given":"R L","family":"Jarvest","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Lowe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/199\/2\/447\/576066\/bj1990447.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/199\/2\/447\/576066\/bj1990447.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T14:23:09Z","timestamp":1637850189000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/199\/2\/447\/18057\/The-stereochemical-course-of-hydrolysis-catalysed"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,11,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1981,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1990447","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1981,11,1]]}}}