{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:56:07Z","timestamp":1791194167746,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,7,1]]},"abstract":"<jats:p>Human cartilage glycoprotein 39 (HC-gp39) is a glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Increased levels of HC-gp39 have been demonstrated in synovial fluids of patients with rheumatoid or osteoarthritis. The increased secretion of HC-gp39 under physiological and pathological conditions with elevated connective-tissue turnover suggests its involvement in the homoeostasis of these tissues. We report here that HC-gp39 promotes the growth of human synovial cells as well as skin and fetal lung fibroblasts. A dose-dependent growth stimulation was observed when each of the fibroblastic cell lines was exposed to HC-gp39 in a concentration range from 0.1 to 2nM, which is similar to the effective dose of the well-characterized mitogen, insulin-like growth factor-1. At suboptimal concentrations, the two growth factors work in a synergistic fashion. The use of selective inhibitors of the mitogen-activated protein kinase and the protein kinase B (AKT) signalling pathways indicates that both are involved in mediating the mitogenic response to HC-gp39. Phosphorylation of both extracellular signal-regulated kinases 1\/2 and AKT occurred in a dose- and time-dependent fashion upon addition of HC-gp39. Activation of these signalling pathways could also be demonstrated in human chondrocytes. Thus HC-gp39 initiates a signalling cascade in connective-tissue cells which leads to increased cell proliferation, suggesting that this protein could play a major role in the pathological conditions leading to tissue fibrosis.<\/jats:p>","DOI":"10.1042\/bj20020075","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T17:38:53Z","timestamp":1027877933000},"page":"119-126","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":326,"title":["The chitinase 3-like protein human cartilage glycoprotein 39 (HC-gp39) stimulates proliferation of human connective-tissue cells and activates both extracellular signal-regulated kinase- and protein kinase B-mediated signalling pathways"],"prefix":"10.1042","volume":"365","author":[{"given":"Anneliese D.","family":"RECKLIES","sequence":"first","affiliation":[{"name":"Joint Diseases Laboratory, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, QC, Canada H3G 1A6"},{"name":"Department of Surgery, McGill University, Montreal, QC, Canada H3G 1Y6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"WHITE","sequence":"additional","affiliation":[{"name":"Joint Diseases Laboratory, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, QC, Canada H3G 1A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"LING","sequence":"additional","affiliation":[{"name":"Joint Diseases Laboratory, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, QC, Canada H3G 1A6"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/365\/1\/119\/709214\/bj3650119.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/365\/1\/119\/709214\/bj3650119.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T07:11:25Z","timestamp":1637565085000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/365\/1\/119\/40125\/The-chitinase-3-like-protein-human-cartilage"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20020075","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,7,1]]}}}