{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T13:24:15Z","timestamp":1773667455541,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,12,1]]},"abstract":"<jats:p>No effects of gaseous NO added at a pressure of 19.95kPa on the stability of the binuclear iron\u2014sulphur centre (ISC) of reduced iron\u2014sulphur protein adrenodoxin (0.2mM) have been observed using the EPR method. However, the incubation of the protein with NO in the presence of ferrous iron (1.8mM) led to complete ISC degradation, accompanied by the formation of protein-bound dinitrosyl iron complexes (DNICs; 0.3\u00b10.1mM). Similar results were obtained when low-molecular-mass DNIC with phosphate or cysteine (1.8mM) were added to solutions of pre-reduced adrenodoxin. The degradation of the ISC was suggested to be due to the attack of the Fe+(NO+)2 group from low-molecular-mass DNICs added or formed during the interaction between NO and ferrous ions on the thiol groups in active centres of adrenodoxin. This attack leads to a release of endogenous iron from the centres, which is capable of forming both low-molecular-mass and protein-bound DNIC, thereby ensuring further ISC degradation.<\/jats:p>","DOI":"10.1042\/bj20020788","type":"journal-article","created":{"date-parts":[[2002,11,27]],"date-time":"2002-11-27T10:42:28Z","timestamp":1038393748000},"page":"633-639","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Exogenous ferrous iron is required for the nitric oxide-catalysed destruction of the iron\u2013sulphur centre in adrenodoxin"],"prefix":"10.1042","volume":"368","author":[{"given":"Nina V.","family":"VOEVODSKAYA","sequence":"first","affiliation":[{"name":"Institute of Chemical Physics, Russian Academy of Sciences, Kosygin Str. 4, Moscow, 119991, Russia,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir A.","family":"SEREZHENKOV","sequence":"additional","affiliation":[{"name":"Institute of Chemical Physics, Russian Academy of Sciences, Kosygin Str. 4, Moscow, 119991, Russia,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris E.","family":"COOPER","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Essex, Central Campus, Wivenhoe Park, Colchester CO4 3SQ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lioudmila N.","family":"KUBRINA","sequence":"additional","affiliation":[{"name":"Institute of Chemical Physics, Russian Academy of Sciences, Kosygin Str. 4, Moscow, 119991, Russia,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anatoly F.","family":"VANIN","sequence":"additional","affiliation":[{"name":"Institute of Chemical Physics, Russian Academy of Sciences, Kosygin Str. 4, Moscow, 119991, Russia,"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/368\/2\/633\/709920\/bj3680633.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/368\/2\/633\/709920\/bj3680633.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:07:41Z","timestamp":1637582861000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/368\/2\/633\/40039\/Exogenous-ferrous-iron-is-required-for-the-nitric"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20020788","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12,1]]}}}