{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T21:43:38Z","timestamp":1789854218956,"version":"4.0.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:p>Matrilin-2 is a component of extracellular filamentous networks. To study the interactions by which it can be integrated into such assemblies, full-length and truncated forms of matrilin-2 were recombinantly expressed in HEK-293 cells and purified from conditioned medium. The recombinant proteins, when used in interaction assays, showed affinity to matrilin-2 itself, but also to other collagenous and non-collagenous extracellular matrix proteins. The interaction between matrilin-2 and collagen I was studied in greater detail and could be shown to occur at distinct sites on the collagen I molecule and to have a KD of about 3\u00d710-8M. Interactions with some non-collagenous protein ligands were even stronger, with matrilin-2 binding to fibrillin-2, fibronectin and laminin-1\u2014nidogen-1 complexes, with KD values in the range of 10-8\u201410-11M. Co-localization of matrilin-2 with these ligands in the dermal-epidermal basement membrane, in the microfibrils extending from the basement membrane into the dermis, and in the dermal extracellular matrix, indicates a physiological relevance of the interactions in the assembly of supramolecular extracellular matrix structures.<\/jats:p>","DOI":"10.1042\/bj20021069","type":"journal-article","created":{"date-parts":[[2002,10,25]],"date-time":"2002-10-25T09:57:42Z","timestamp":1035539862000},"page":"715-721","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Matrilin-2 interacts with itself and with other extracellular matrix proteins"],"prefix":"10.1042","volume":"367","author":[{"given":"Dorothea","family":"PIECHA","sequence":"first","affiliation":[{"name":"Institute for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"WIBERG","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, BMC\/C12, University of Lund, S-221 84 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"M\u00d6RGELIN","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, BMC\/C12, University of Lund, S-221 84 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter P.","family":"REINHARDT","sequence":"additional","affiliation":[{"name":"Institute for Medical Molecular Biology, Medical University of L\u00fcbeck, D-23538 L\u00fcbeck, Germany,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferenc","family":"DE\u00c1K","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrik","family":"MAURER","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mats","family":"PAULSSON","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/367\/3\/715\/709866\/bj3670715.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/367\/3\/715\/709866\/bj3670715.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:24:05Z","timestamp":1637583845000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/367\/3\/715\/40423\/Matrilin-2-interacts-with-itself-and-with-other"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20021069","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11,1]]}}}