{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:46:03Z","timestamp":1777542363862,"version":"3.51.4"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,4,1]]},"abstract":"<jats:p>The T-allele of a polymorphism (C825T) in the gene for the G-protein \u03b23 subunit (GNB3) is associated with cardiovascular and metabolic disorders, distinct cellular features and altered drug responses. The molecular mechanisms that give rise to this complex phenotype have been linked to the occurrence of G\u03b23s, a splice variant of GNB3. G\u03b23s is predominantly expressed in cells with the 825T-allele. In the present study we describe the identification and characterization of an additional G\u03b23 splice variant referred to as G\u03b23s2. Its mRNA is expressed in heart, blood cells and tumour tissue, and its expression is also tightly associated with the GNB3 825T-allele. G\u03b23s2 is generated by alternative splicing using non-canonical splice sites. G\u03b2 subunits belong to the family of propeller proteins and consist of seven regular propeller blades. Transcripts for G\u03b23s2 are lacking 129bp of the coding sequence of the wild-type G\u03b23 protein. Thus the predicted structure consists of only six propeller blades, which resembles the structure of G\u03b23s. Co-immunoprecipitation analyses indicated that G\u03b23s2 dimerizes with different G\u03b3 subunits, e.g. G\u03b35, G\u03b38C and G\u03b312. In Sf9 insect cells, expression of G\u03b23s2 together with G\u03b312 enhances receptor-stimulated activation of G\u03b1i2. Expression of G\u03b23s2 in mammalian cells activated the mitogen-activated protein kinase cascade. Together, these results suggest that G\u03b23s2 is a biologically active G\u03b2 variant which may play a role in the manifestation of the complex phenotype associated with the 825T-allele.<\/jats:p>","DOI":"10.1042\/bj20021208","type":"journal-article","created":{"date-parts":[[2003,3,26]],"date-time":"2003-03-26T14:27:08Z","timestamp":1048688828000},"page":"223-232","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Identification and characterization of Gbeta3s2, a novel splice variant of the G-protein beta3 subunit"],"prefix":"10.1042","volume":"371","author":[{"given":"Dieter","family":"ROSSKOPF","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris","family":"MANTHEY","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"HABICH","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marzena","family":"KIELBIK","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"EISENHARDT","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"NIKULA","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"URBAN","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"KOHNEN","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"GRAF","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie and Toxikologie, Medizinische Fakult\u00e4t, Carl Gustav Carus, Technische Universit\u00e4t, D-01307 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"RAVENS","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie and Toxikologie, Medizinische Fakult\u00e4t, Carl Gustav Carus, Technische Universit\u00e4t, D-01307 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Winfried","family":"SIFFERT","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie, Universit\u00e4tsklinikum Essen, D-45122 Essen, Germany,"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/371\/1\/223\/710846\/bj3710223.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/371\/1\/223\/710846\/bj3710223.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:05:05Z","timestamp":1637582705000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/371\/1\/223\/39974\/Identification-and-characterization-of-Gbeta3s2-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,4,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20021208","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4,1]]}}}