{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:41:01Z","timestamp":1791117661835,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,6,15]]},"abstract":"<jats:p>We report the isolation and amino acid sequences of six novel dimeric disintegrins from the venoms of Vipera lebetina obtusa (VLO), V. berus (VB), V. ammodytes (VA), Echis ocellatus (EO) and Echis multisquamatus (EMS). Disintegrins VLO4, VB7, VA6 and EO4 displayed the RGD motif and inhibited the adhesion of K562 cells, expressing the integrin \u03b15\u03b21 to immobilized fibronectin. A second group of dimeric disintegrins (VLO5 and EO5) had MLD and VGD motifs in their subunits and blocked the adhesion of the \u03b14\u03b21 integrin to vascular cell adhesion molecule 1 with high selectivity. On the other hand, disintegrin EMS11 inhibited both \u03b15\u03b21 and \u03b14\u03b21 integrins with almost the same degree of specificity. Comparison of the amino acid sequences of the dimeric disintegrins with those of other disintegrins by multiple-sequence alignment and phylogenetic analysis, in conjunction with current biochemical and genetic data, supports the view that the different disintegrin subfamilies evolved from a common ADAM (adisintegrin and metalloproteinase-like) scaffold and that structural diversification occurred through disulphide bond engineering.<\/jats:p>","DOI":"10.1042\/bj20021739","type":"journal-article","created":{"date-parts":[[2003,6,9]],"date-time":"2003-06-09T13:24:00Z","timestamp":1055165040000},"page":"725-734","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":164,"title":["Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering"],"prefix":"10.1042","volume":"372","author":[{"given":"Juan J.","family":"CALVETE","sequence":"first","affiliation":[{"name":"Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Paz","family":"MORENO-MURCIANO","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. David G.","family":"THEAKSTON","sequence":"additional","affiliation":[{"name":"Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dariusz G.","family":"KISIEL","sequence":"additional","affiliation":[{"name":"Biotechnology Center, Temple University College of Science and Technology, 1900 N. 12th Street, Philadelphia, PA 19122-6078, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cezary","family":"MARCINKIEWICZ","sequence":"additional","affiliation":[{"name":"Biotechnology Center, Temple University College of Science and Technology, 1900 N. 12th Street, Philadelphia, PA 19122-6078, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/372\/3\/725\/710927\/bj3720725.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/372\/3\/725\/710927\/bj3720725.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:20:02Z","timestamp":1637583602000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/372\/3\/725\/40328\/Snake-venom-disintegrins-novel-dimeric"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,6,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,6,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20021739","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,6,15]]}}}