{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:03:10Z","timestamp":1791291790180,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,4,15]]},"abstract":"<jats:p>HS1 (haematopoietic lineage cell-specific gene protein 1), a prominent substrate of intracellular protein tyrosine kinases in haematopoietic cells, is implicated in the immune response to extracellular stimuli and in cell differentiation induced by cytokines. Although HS1 contains a 37-amino acid tandem repeat motif and a C-terminal Src homology 3 domain and is closely related to the cortical-actin-associated protein cortactin, it lacks the fourth repeat that has been shown to be essential for cortactin binding to filamentous actin (F-actin). In this study, we examined the possible role of HS1 in the regulation of the actin cytoskeleton. Immunofluorescent staining demonstrated that HS1 co-localizes in the cytoplasm of cells with actin-related protein (Arp) 2\/3 complex, the primary component of the cellular machinery responsible for de novo actin assembly. Furthermore, recombinant HS1 binds directly to Arp2\/3 complex with an equilibrium dissociation constant (Kd) of 880nM. Although HS1 is a modest F-actin-binding protein with a Kd of 400nM, it increases the rate of the actin assembly mediated by Arp2\/3 complex, and promotes the formation of branched actin filaments induced by Arp2\/3 complex and a constitutively activated peptide of N-WASP (neural Wiskott\u2013Aldrich syndrome protein). Our data suggest that HS1, like cortactin, plays an important role in the modulation of actin assembly.<\/jats:p>","DOI":"10.1042\/bj20021791","type":"journal-article","created":{"date-parts":[[2003,4,9]],"date-time":"2003-04-09T05:34:54Z","timestamp":1049866494000},"page":"485-493","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Haematopoietic lineage cell-specific protein 1 (HS1) promotes actin-related protein (Arp) 2\/3 complex-mediated actin polymerization"],"prefix":"10.1042","volume":"371","author":[{"given":"Takehito","family":"URUNO","sequence":"first","affiliation":[{"name":"Department of Experimental Pathology, Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peijun","family":"ZHANG","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, U.S.A."},{"name":"Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"LIU","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Jiang","family":"HAO","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"ZHAN","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, U.S.A."},{"name":"Department of Cell Biology and Anatomy, The George Washington University, Washington, DC 20001, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/371\/2\/485\/711219\/bj3710485.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/371\/2\/485\/711219\/bj3710485.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T07:40:24Z","timestamp":1637566824000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/371\/2\/485\/40844\/Haematopoietic-lineage-cell-specific-protein-1-HS1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,4,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20021791","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4,15]]}}}