{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T15:23:34Z","timestamp":1783092214392,"version":"3.54.6"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,7,15]]},"abstract":"<jats:p>Nucleoside monophosphate kinases (NMPKs) are essential catalysts for bacterial growth and multiplication. These enzymes display high primary sequence identities among members of the family Enterobacteriaceae. Yersinia pestis, the causative agent of plague, belongs to this family. However, it was previously shown that its thymidylate kinase (TMPKyp) exhibits biochemical properties significantly different from those of its Escherichia coli counterpart [Chenal-Francisque, Tourneux, Carniel, Christova, Li de la Sierra, Barzu and Gilles (1999) Eur. J. Biochem. 265, 112\u2013119]. In this work, the adenylate kinase (AK) of Y. pestis (AKyp) was characterized. As with TMPKyp, AKyp displayed a lower thermodynamic stability than other studied AKs. Two mutations in AK (Ser129\u2192Phe and Pro87\u2192Ser), previously shown to induce a thermosensitive growth defect in E. coli, were introduced into AKyp. The recombinant variants had a lower stability than wild-type AKyp and a higher susceptibility to proteolytic digestion. When the Pro87\u2192Ser substitution was introduced into the chromosomal adk gene of Y. pestis, growth of the mutant strain was altered at the non-permissive temperature of 37 \u00b0C. In virulence testings, less than 50 colony forming units (CFU) of wild-type Y. pestis killed 100% of the mice upon subcutaneous infection, whereas bacterial loads as high as 1.5\u00d7104 CFU of the adk mutant were unable to kill any animals.<\/jats:p>","DOI":"10.1042\/bj20030284","type":"journal-article","created":{"date-parts":[[2003,7,11]],"date-time":"2003-07-11T10:35:08Z","timestamp":1057919708000},"page":"515-522","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Relationship between bacterial virulence and nucleotide metabolism: a mutation in the adenylate kinase gene renders Yersinia pestis avirulent"],"prefix":"10.1042","volume":"373","author":[{"given":"H\u00e9l\u00e8ne","family":"MUNIER-LEHMANN","sequence":"first","affiliation":[{"name":"Laboratoire de Chimie Structurale des Macromol\u00e9cules, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane","family":"CHENAL-FRANCISQUE","sequence":"additional","affiliation":[{"name":"Laboratoire des Yersinia, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela","family":"IONESCU","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Structurale des Macromol\u00e9cules, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petya","family":"CHRISTOVA","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Structurale des Macromol\u00e9cules, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeannine","family":"FOULON","sequence":"additional","affiliation":[{"name":"Laboratoire des Yersinia, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"CARNIEL","sequence":"additional","affiliation":[{"name":"Laboratoire des Yersinia, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Octavian","family":"B\u00c2RZU","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Structurale des Macromol\u00e9cules, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/373\/2\/515\/711352\/bj3730515.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/373\/2\/515\/711352\/bj3730515.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:35:02Z","timestamp":1637584502000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/373\/2\/515\/40679\/Relationship-between-bacterial-virulence-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,7,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20030284","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7,15]]}}}