{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:37:40Z","timestamp":1790980660159,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,1,15]]},"abstract":"<jats:p>Application of reverse transcription\u2013PCR to total RNA prepared from TNF-\u03b1 (tumour necrosis factor-\u03b1)-stimulated HUVECs (human umbilical vein endothelial cells) revealed that the syndecan-2 mRNA was up-regulated by this inflammatory stimulus. By immunoprecipitation using an anti-syndecan-2 antibody on TNF-\u03b1-stimulated HUVEC lysates, inflammation-induced interleukin-8 was found to be an interaction partner of this HS (heparan sulphate) proteoglycan, but not of any other syndecan on these cells. The glycosylated [Syn2ect(+HS)] and non-glycosylated [Syn2ect(\u2212HS)] forms of Syn2ect (the syndecan-2 ectodomain) were purified from a stably transfected human cell line and from a bacterial expression system respectively. By CD spectroscopy, Syn2ect was found to adopt an all-\u03b2 secondary structure. The dissociation constant of Syn2ect(+HS) with respect to interleukin-8 binding was determined by isothermal fluorescence titrations to be 23 nM. Despite its lack of HS chains, Syn2ect(\u2212HS) exhibited significant binding to the chemokine, with a Kd of &amp;gt;1 \u00b5M. Thus, in addition to glycosaminoglycan binding, protein\u2013protein contacts might also contribute to the chemokine\u2013proteoglycan interaction.<\/jats:p>","DOI":"10.1042\/bj20030729","type":"journal-article","created":{"date-parts":[[2004,1,12]],"date-time":"2004-01-12T15:01:33Z","timestamp":1073919693000},"page":"533-538","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Interleukin-8 binds to syndecan-2 on human endothelial cells"],"prefix":"10.1042","volume":"377","author":[{"given":"Yvonne","family":"HALDEN","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, University of Graz, Universit\u00e4tsplatz 1, A-8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"REK","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, University of Graz, Universit\u00e4tsplatz 1, A-8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"ATZENHOFER","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, University of Graz, Universit\u00e4tsplatz 1, A-8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laszlo","family":"SZILAK","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, University of Graz, Universit\u00e4tsplatz 1, A-8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"WABNIG","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, University of Graz, Universit\u00e4tsplatz 1, A-8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas J.","family":"KUNGL","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, University of Graz, Universit\u00e4tsplatz 1, A-8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/377\/2\/533\/712689\/bj3770533.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/377\/2\/533\/712689\/bj3770533.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:46:02Z","timestamp":1637585162000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/377\/2\/533\/40975\/Interleukin-8-binds-to-syndecan-2-on-human"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,1,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20030729","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1,15]]}}}