{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:28:15Z","timestamp":1790911695623,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,2,1]]},"abstract":"<jats:p>The intracellular pathogen Burkholderia pseudomallei is the causative agent of tropical melioidosis, and Burkholderia thailandensis is a closely-related Gram-negative bacterium that does not cause serious disease. Like other bacteria, the major outer membrane (OM) porins of Burkholderia strains, BpsOmp38 and BthOmp38 may have roles in antibiotic resistance and immunity. We purified both proteins and found them to be immunologically related, SDS-resistant, heat-sensitive trimers with Mr of approx. 110000. In functional liposome-swelling assays, both proteins showed similar permeabilities for small sugar molecules, compatible with a pore diameter of between 1.2 and 1.6 nm. Secondary structure analysis by FTIR (Fourier-transform infrared) spectroscopy revealed almost identical spectra with predominantly \u03b2-sheet structures, typical of bacterial porins. MALDI\u2013TOF (matrix-assisted laser-desorption ionization\u2013time of flight) MS and ESI\/MS (electrospray ionization MS) analysis of each protein showed extensive sequence similarities to the OpcP1 porin from Burkholderia cepacia (later found to be 76.5% identical). Based on information from the incomplete B. pseudomallei genome-sequencing project, the genes encoding Omp38 were identified and amplified by PCR from B. pseudomallei and B. thailandensis genomic DNA. The nucleotide sequences are 99.7% identical, and the predicted processed proteins are 100% identical. Topology prediction and molecular modelling suggest that this newly-isolated and cloned porin is a 16-stranded \u03b2-barrel and the external loops of the protein could be important determinants of the immune response to infection.<\/jats:p>","DOI":"10.1042\/bj20031118","type":"journal-article","created":{"date-parts":[[2007,11,7]],"date-time":"2007-11-07T15:30:58Z","timestamp":1194449458000},"page":"579-587","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Functional reconstitution, gene isolation and topology modelling of porins from Burkholderia pseudomallei and Burkholderia thailandensis"],"prefix":"10.1042","volume":"377","author":[{"given":"Jaruwan","family":"SIRITAPETAWEE","sequence":"first","affiliation":[{"name":"School of Biochemistry, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heino","family":"PRINZ","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut f\u00fcr Molekulare Physiologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Worada","family":"SAMOSORNSUK","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Faculty of Allied Health Science, Thammasat University, Pathumthani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard H.","family":"ASHLEY","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Edinburgh, George Square, Edinburgh EH8 9XD, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wipa","family":"SUGINTA","sequence":"additional","affiliation":[{"name":"School of Biochemistry, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/377\/3\/579\/712933\/bj3770579.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/377\/3\/579\/712933\/bj3770579.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:49:46Z","timestamp":1637585386000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/377\/3\/579\/41092\/Functional-reconstitution-gene-isolation-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,2,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20031118","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2,1]]}}}