{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:25:47Z","timestamp":1791606347206,"version":"4.3.4"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,3,15]]},"abstract":"<jats:p>A novel HERG channel blocker was isolated from the venom of the scorpion Buthus martensi Karsch, sequenced and characterized at the pharmacological level after chemical synthesis. According to the determined amino acid sequence, the cDNA and genomic genes were then cloned. The genomic gene consists of two exons interrupted by an intron of 65 bp at position \u22126 upstream from the mature toxin. The protein sequence of this toxin was completely identical with that of a known A-type K+ current blocker BmTx3, belonging to scorpion \u03b1-KTx subfamily 15. Thus BmTx3 is the first reported \u03b1-KTx peptide also showing HERG-blocking activity, like \u03b3-KTx peptides. Moreover, different from classical \u03b1-KTx peptides, such as charybdotoxin, BmTx3 cannot block Shaker-type K+ channels. Phylogenetic tree analysis reveals that this toxin takes an intermediate position between classical \u03b1-KTx and \u03b3-KTx toxins. From a structural point of view, we propose that two separate functional faces might exist on the BmTx3 molecule, responsible for the two different K+-current-blocking functions. Face A, composed of Arg18 and Lys19 in the \u03b1-helix side, might correspond to HERG blocking activity, whereas Face B, containing a putative functional dyad (Lys27 and Tyr36) in the \u03b2-sheet side, might correspond to A-type blocking activity. A specific deletion mutant with the disrupted Face B, BmTx3-Y36P37del, loses the A-type current-blocking activity, but keeps a similar HERG-blocking activity, as seen with the wild-type toxin.<\/jats:p>","DOI":"10.1042\/bj20031324","type":"journal-article","created":{"date-parts":[[2004,4,13]],"date-time":"2004-04-13T15:10:17Z","timestamp":1081869017000},"page":"745-752","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["BmTx3, a scorpion toxin with two putative functional faces separately active on A-type K+ and HERG currents"],"prefix":"10.1042","volume":"378","author":[{"given":"Isabelle","family":"HUYS","sequence":"first","affiliation":[{"name":"Laboratory of Toxicology, University of Leuven, Faculty of Pharmaceutical Sciences, E. Van Evenstraat 4, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Qi","family":"XU","sequence":"additional","affiliation":[{"name":"Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Zhong","family":"WANG","sequence":"additional","affiliation":[{"name":"Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China"},{"name":"Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"VACHER","sequence":"additional","affiliation":[{"name":"UMR 6560 CNRS Universit\u00e9 de la Mediterran\u00e9e, Institut Jean Roche, Facult\u00e9 de M\u00e9decine Nord, Boulevard Pierre Dramard, 13916 Marseille Cedex 20, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-France","family":"MARTIN-EAUCLAIRE","sequence":"additional","affiliation":[{"name":"UMR 6560 CNRS Universit\u00e9 de la Mediterran\u00e9e, Institut Jean Roche, Facult\u00e9 de M\u00e9decine Nord, Boulevard Pierre Dramard, 13916 Marseille Cedex 20, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Wu","family":"CHI","sequence":"additional","affiliation":[{"name":"Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China"},{"name":"Institute of Protein Research, Tong Ji University, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"TYTGAT","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology, University of Leuven, Faculty of Pharmaceutical Sciences, E. Van Evenstraat 4, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/378\/3\/745\/733302\/bj3780745.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/378\/3\/745\/733302\/bj3780745.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T13:02:07Z","timestamp":1637586127000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/378\/3\/745\/41509\/BmTx3-a-scorpion-toxin-with-two-putative"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,3,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20031324","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,3,15]]}}}