{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:56:14Z","timestamp":1790675774944,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,3,1]]},"abstract":"<jats:p>KLH (keyhole limpet haemocyanin), the oxygen-carrying molecule of the marine snail Megathura crenulata, is often used as an adjuvant or as a hapten carrier for immunizations with peptides, oligosaccharides or other low-molecular-mass organic compounds. KLH exhibits several carbohydrate determinants, at least some of which are immunogenic: it shares an antigenic Fuc(\u03b11-3)GalNAc-determinant with schistosomes and contains unique Gal-(\u03b21-6)Man-structural motifs on its N-glycans. This study reveals the presence of N-glycans with unusual \u00b1Gal(\u03b21-4)Gal(\u03b21-4)Fuc- units (\u03b11-6)-linked to the reducing end N-acetylglucosamine residue. The following novel structures of KLH N-glycans were deduced by linkage analysis, exoglycosidase digestion, matrix-assisted laser-desorption ionization-tandem MS and nano-LC-ESI-IT-MS (where LC stands for liquid chromatography, ESI for electrospray ionization and IT for ion trap): Man(\u03b11-6)[\u00b1Man(\u03b11-3)]Man(\u03b21-4)GlcNAc(\u03b21-4)[Gal(\u03b21-4)Fuc(\u03b11-6)]GlcNAc and Man(\u03b11-6)Man(\u03b21-4)GlcNAc(\u03b21-4)[Gal(\u03b21-4)Gal(\u03b21-4)Fuc(\u03b11-6)]GlcNAc. The Gal(\u03b21-4)Fuc- and Gal(\u03b21-4)Gal(\u03b21-4)Fuc- core modifications are expected to be immunogenic, similar to other non-mammalian-type core modifications, and to contribute to the immunostimulatory properties of KLH.<\/jats:p>","DOI":"10.1042\/bj20031380","type":"journal-article","created":{"date-parts":[[2004,2,24]],"date-time":"2004-02-24T15:35:09Z","timestamp":1077636909000},"page":"625-632","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["A novel Gal(beta1-4)Gal(beta1-4)Fuc(alpha1-6)-core modification attached to the proximal N-acetylglucosamine of keyhole limpet haemocyanin (KLH) N-glycans"],"prefix":"10.1042","volume":"378","author":[{"given":"Manfred","family":"WUHRER","sequence":"first","affiliation":[{"name":"Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjolein L. M.","family":"ROBIJN","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolien A. M.","family":"KOELEMAN","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crina I. A.","family":"BALOG","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"GEYER","sequence":"additional","affiliation":[{"name":"Medical Faculty, Institute of Biochemistry, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 M.","family":"DEELDER","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelis H.","family":"HOKKE","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/378\/2\/625\/713174\/bj3780625.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/378\/2\/625\/713174\/bj3780625.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:54:00Z","timestamp":1637585640000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/378\/2\/625\/41299\/A-novel-Gal-beta1-4-Gal-beta1-4-Fuc-alpha1-6-core"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20031380","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,3,1]]}}}