{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:12:23Z","timestamp":1791216743109,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,6,15]]},"abstract":"<jats:p>C4.4A, a structural homologue of the urokinase-type plasminogen activator receptor (uPAR), was originally identified as a metastasis-associated membrane protein, but little is known about its structural and functional properties. Therefore, we expressed, purified and characterized a soluble truncated form of human C4.4A, and used this protein to produce specific polyclonal anti-C4.4A antibodies. By immunohistochemistry we observed a pronounced surface staining for C4.4A in suprabasal keratinocytes of chronic human wounds and found C4.4A expression markedly upregulated in migrating keratinocytes during re-epithelisation of incisional skin wounds. Phorbol-ester-induced hyperplasia of mouse skin is also accompanied by a significant induction of C4.4A expression in the multilayered, suprabasal keratinocytes. C4.4A contains two Ly-6 (leucocyte antigen 6)\/uPAR\/\u03b1-neurotoxin modules. Our recombinant human C4.4A is extensively modified by post-translational glycosylation, which include 5\u20136 N-linked carbohydrates primarily located in or close to its second Ly-6\/uPAR\/\u03b1-neurotoxin module and approximately 15 O-linked carbohydrates clustered in a Ser\/Thr\/Pro-rich region at the C-terminus. A highly protease-sensitive region (Tyr200\u2013Arg204) is located between these two clusters of N- and O-linked carbohydrates. The natural, glycolipid-anchored C4.4A from amnion membranes of human term placenta exhibits similar properties. Using recombinant, soluble C4.4A or MCF 7 cells, which express significant amounts of GPI-anchored C4.4A, we find no evidence for an interaction between C4.4A and uPA, a property suggested previously for rat C4.4A. Collectively these data indicate that C4.4A, although being a structural homologue of uPAR, is unlikely to have a functional overlap with uPAR.<\/jats:p>","DOI":"10.1042\/bj20031478","type":"journal-article","created":{"date-parts":[[2004,6,17]],"date-time":"2004-06-17T11:42:25Z","timestamp":1087472545000},"page":"845-857","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Structural analysis and tissue localization of human C4.4A: a protein homologue of the urokinase receptor"],"prefix":"10.1042","volume":"380","author":[{"given":"Line V.","family":"HANSEN","sequence":"first","affiliation":[{"name":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"G\u00c5RDSVOLL","sequence":"additional","affiliation":[{"name":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boye S.","family":"NIELSEN","sequence":"additional","affiliation":[{"name":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leif R.","family":"LUND","sequence":"additional","affiliation":[{"name":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keld","family":"DAN\u00d8","sequence":"additional","affiliation":[{"name":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole N.","family":"JENSEN","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"PLOUG","sequence":"additional","affiliation":[{"name":"Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/380\/3\/845\/714981\/bj3800845.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/380\/3\/845\/714981\/bj3800845.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T14:54:28Z","timestamp":1637592868000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/380\/3\/845\/43981\/Structural-analysis-and-tissue-localization-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,6,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20031478","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6,15]]}}}