{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:29:46Z","timestamp":1791307786800,"version":"4.3.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,3,1]]},"abstract":"<jats:p>Platelet glycoprotein Ib (GPIb)-binding proteins (GPIb-BPs) from snake venoms are usually C-type lectins, which target specific sites of GPIb\u03b1 and elicit distinct effects on platelets. In the present paper, we report a tetrameric platelet-agglutinating factor (molecular mass 121.1 kDa), termed mucrocetin, purified from the venom of Taiwan habu (Trimeresurus mucrosquamatus). Mucrocetin is a GPIb\u03b1 agonist with a binding site distinct from that of flavocetin-A (a snake venom GPIb\u03b1 antagonist) on GPIb\u03b1, in spite of the high sequence identity (94.6%) between the two venom lectins. The crystal structure of mucrocetin was solved and refined to 2.8 \u00c5 (1 \u00c5=0.1 nm) resolution, which shows an interesting crystal packing of six-layer cylinders of doughnut-shaped molecules. The four \u03b1\u03b2 heterodimers are arranged in an unusual square-shaped ring stabilized by four interdimer \u2018head-to-tail\u2019 disulphide bridges. Detailed structural comparison between mucrocetin and flavocetin-A suggests that their disparate platelet effects are probably attributable to different charge distributions on the putative concave binding surface. A unique positively charged patch on the binding surface of mucrocetin, formed by Lys102, Lys108, Lys109 and Arg123 in the \u03b1-subunit coupled with Lys22, Lys102, Lys116 and Arg117 in the \u03b2-subunit, appears to be the primary determinant of its platelet-agglutinating activity. Conceivably, this interesting venom factor may provide a useful tool to study platelet agglutination by binding to the GPIb\u2013IX\u2013V complex.<\/jats:p>","DOI":"10.1042\/bj20031507","type":"journal-article","created":{"date-parts":[[2004,2,24]],"date-time":"2004-02-24T15:35:09Z","timestamp":1077636909000},"page":"399-407","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Crystal structure of a platelet-agglutinating factor isolated from the venom of Taiwan habu (Trimeresurus mucrosquamatus)"],"prefix":"10.1042","volume":"378","author":[{"given":"Kai-Fa","family":"HUANG","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan"},{"name":"Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Ping","family":"KO","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Chun","family":"HUNG","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"CHU","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew H.-J.","family":"WANG","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan"},{"name":"Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyh-Horng","family":"CHIOU","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 11529, Taiwan"},{"name":"Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/378\/2\/399\/713164\/bj3780399.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/378\/2\/399\/713164\/bj3780399.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:52:42Z","timestamp":1637585562000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/378\/2\/399\/41267\/Crystal-structure-of-a-platelet-agglutinating"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20031507","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,3,1]]}}}