{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:43:54Z","timestamp":1791200634240,"version":"4.2.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,4,15]]},"abstract":"<jats:p>It is now thought that NO\u2022 (nitric oxide) and its redox congeners may play a role in the physiological regulation of mitochondrial function. The inhibition of cytochrome c oxidase by NO\u2022 is characterized as being reversible and oxygen dependent. In contrast, peroxynitrite, the product of the reaction of NO\u2022 with superoxide, irreversibly inhibits several of the respiratory complexes. However, little is known about the effects of HNO (nitroxyl) on mitochondrial function. This is especially important, since HNO has been shown to be more cytotoxic than NO\u2022, may potentially be generated in vivo, and elicits biological responses with some of the characteristics of NO and peroxynitrite. In the present study, we present evidence that isolated mitochondria, in the absence or presence of substrate, convert HNO into NO\u2022 by a process that is dependent on mitochondrial concentration as well as the concentration of the HNO donor Angeli's salt. In addition, HNO is able to inhibit mitochondrial respiration through the inhibition of complexes I and II, most probably via modification of specific cysteine residues in the proteins. Using a proteomics approach, extensive modification of mitochondrial protein thiols was demonstrated. From these data it is evident that HNO interacts with mitochondria through mechanisms distinct from those of either NO\u2022 or peroxynitrite, including the generation of NO\u2022, the modification of thiols and the inhibition of complexes I and II.<\/jats:p>","DOI":"10.1042\/bj20031758","type":"journal-article","created":{"date-parts":[[2004,4,13]],"date-time":"2004-04-13T15:19:29Z","timestamp":1081869569000},"page":"359-366","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Mechanisms of the interaction of nitroxyl with mitochondria"],"prefix":"10.1042","volume":"379","author":[{"given":"Sruti","family":"SHIVA","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack H.","family":"CRAWFORD","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anup","family":"RAMACHANDRAN","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin K.","family":"CEASER","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tess","family":"HILLSON","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul S.","family":"BROOKES","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."},{"name":"Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh P.","family":"PATEL","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."},{"name":"Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor M.","family":"DARLEY-USMAR","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."},{"name":"Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/379\/2\/359\/713318\/bj3790359.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/379\/2\/359\/713318\/bj3790359.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T12:52:37Z","timestamp":1637585557000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/379\/2\/359\/41264\/Mechanisms-of-the-interaction-of-nitroxyl-with"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,4,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20031758","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4,15]]}}}