{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:53:25Z","timestamp":1790553205501,"version":"4.1.0"},"reference-count":26,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,7,1]]},"abstract":"<jats:p>Extracts of frozen rat liver were found to catalyse the formation of 3H2O from DL-2-hydroxy[2-3H]glutarate. Three peaks of enzyme activities were observed on separation by chromatography on DEAE-Sepharose. The first and second peaks corresponded to an enzyme acting on L-2-hydroxyglutarate and the third peak corresponded to an enzyme acting on D-2-hydroxyglutarate, as indicated by competitive inhibition of the detritiation of the racemic radioactive compound by the unlabelled L- and D-isomers respectively. The enzyme acting on the D-form was further characterized. It was independent of NAD or NADP and it converted D-2-hydroxyglutarate into \u03b1-ketoglutarate, transferring electrons to artificial electron acceptors. It also oxidized D-lactate, D-malate and meso-tartrate and was stimulated by Zn2+, Co2+ and Mn2+, but not by Mg2+ or Ca2+. Subcellular fractionation indicated that it was present in the mitochondrial fraction. The enzyme was further purified by chromatography on Blue Trisacryl and phenyl-Sepharose, up to a stage where only a few bands were still visible by SDS\/ PAGE. Among the four candidate polypeptides that were identified by MS, one corresponded to a predicted mitochondrial protein homologous with FAD-dependent D-lactate dehydrogenase. The corresponding human protein was expressed in HEK-293\u00a0cells and it was shown to catalyse the detritiation of DL-2-hydroxy[2-3H]glutarate with similar properties as the purified rat enzyme.<\/jats:p>","DOI":"10.1042\/bj20031933","type":"journal-article","created":{"date-parts":[[2004,6,22]],"date-time":"2004-06-22T09:45:11Z","timestamp":1087897511000},"page":"35-42","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":121,"title":["Identification of a dehydrogenase acting on\n                    <scp>D<\/scp>\n                    -2-hydroxyglutarate"],"prefix":"10.1042","volume":"381","author":[{"given":"Younes","family":"ACHOURI","sequence":"first","affiliation":[{"name":"Laboratory of Physiological Chemistry, Universit\u00e9 catholique de Louvain and Christian de Duve Institute of Cellular Pathology (ICP), Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebtane","family":"NO\u00cbL","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Chemistry, Universit\u00e9 catholique de Louvain and Christian de Duve Institute of Cellular Pathology (ICP), Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"VERTOMMEN","sequence":"additional","affiliation":[{"name":"Hormone and Metabolic Research Unit, Universit\u00e9 catholique de Louvain and Christian de Duve Institute of Cellular Pathology (ICP), Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark\u00a0H.","family":"RIDER","sequence":"additional","affiliation":[{"name":"Hormone and Metabolic Research Unit, Universit\u00e9 catholique de Louvain and Christian de Duve Institute of Cellular Pathology (ICP), Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"VEIGA-DA-CUNHA","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Chemistry, Universit\u00e9 catholique de Louvain and Christian de Duve Institute of Cellular Pathology (ICP), Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emile","family":"van SCHAFTINGEN","sequence":"additional","affiliation":[{"name":"Laboratory of Physiological Chemistry, Universit\u00e9 catholique de Louvain and Christian de Duve Institute of Cellular Pathology (ICP), Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2004,6,22]]},"reference":[{"key":"2021112213003379200_B1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/BF02312516","article-title":"D-2-hydroxyglutaric aciduria: case report and biochemical studies","volume":"3","author":"Chalmers","year":"1980","journal-title":"J. Inherit. Metab. Dis."},{"key":"2021112213003379200_B2","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1023\/A:1005548005393","article-title":"D-2-hydroxyglutaric aciduria: further clinical delineation","volume":"22","author":"van der Knaap","year":"1999","journal-title":"J. Inherit. Metab. Dis."},{"key":"2021112213003379200_B3","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/0003-9861(67)90463-8","article-title":"Metabolism of 2-amino-5-hydroxyadipic acid in the rat","volume":"119","author":"Lindahl","year":"1967","journal-title":"Arch. Biochem. Biophys."},{"key":"2021112213003379200_B4","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/BF00711764","article-title":"D-2-hydroxyglutaric acidaemia: identification of a new enzyme, D-2-hydroxyglutarate dehydrogenase, localized in mitochondria","volume":"18","author":"Wanders","year":"1995","journal-title":"J. Inherit. Metab. Dis."},{"key":"2021112213003379200_B5","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/0006-291X(60)90166-2","article-title":"Effects of metal-complexing agents on mitochondrial D-\u03b1-hydroxy acid dehydrogenase","volume":"3","author":"Tubbs","year":"1960","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021112213003379200_B6","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1042\/bj0810104","article-title":"The oxidation of D-\u03b1-hydroxy acids in animal tissues","volume":"81","author":"Tubbs","year":"1961","journal-title":"Biochem. J."},{"key":"2021112213003379200_B7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1042\/bj1150055","article-title":"Assay, purification and properties of mammalian D-2-hydroxy acid dehydrogenase","volume":"115","author":"Cammack","year":"1969","journal-title":"Biochem. J."},{"key":"2021112213003379200_B8","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.2337\/diabetes.49.10.1627","article-title":"Identification, cloning and heterologous expression of a mammalian fructosamine-3-kinase","volume":"49","author":"Delpierre","year":"2000","journal-title":"Diabetes"},{"key":"2021112213003379200_B9","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1128\/IAI.71.1.242-253.2003","article-title":"Identification of substrates and chaperone from the Yersinia enterocolitica 1B Ysa type III secretion system","volume":"71","author":"Foultier","year":"2003","journal-title":"Infect. Immun."},{"key":"2021112213003379200_B10","first-page":"20","article-title":"2-Oxoglutarate","volume-title":"Methods of Enzymatic Analysis","author":"Burlina","year":"1986","edition":"3rd edn"},{"key":"2021112213003379200_B11","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/0003-2697(75)90577-1","article-title":"A spectrophotometric procedure for determining the activity of various rat tissue oxidases","volume":"69","author":"Duley","year":"1975","journal-title":"Anal. Biochem."},{"key":"2021112213003379200_B12","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1042\/bj0600604","article-title":"Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat liver tissue","volume":"60","author":"de Duve","year":"1955","journal-title":"Biochem. J."},{"key":"2021112213003379200_B13","doi-asserted-by":"crossref","first-page":"7297","DOI":"10.1073\/pnas.92.16.7297","article-title":"A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethyleneimine","volume":"92","author":"Boussif","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021112213003379200_B14","doi-asserted-by":"crossref","first-page":"7580","DOI":"10.1128\/JB.181.24.7580-7587.1999","article-title":"The Archaeoglobus fulgidus D-lactate dehydrogenase is a Zn2+ flavoprotein","volume":"181","author":"Reed","year":"1999","journal-title":"J. Bacteriol."},{"key":"2021112213003379200_B15","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/S0006-291X(02)00768-4","article-title":"Identification of putative mammalian D-lactate dehydrogenase enzymes","volume":"295","author":"Flick","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021112213003379200_B16","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1006\/jmbi.2000.3903","article-title":"Predicting subcellular localization of proteins based on their N-terminal amino acid sequence","volume":"300","author":"Emanuelsson","year":"2000","journal-title":"J. Mol. Biol."},{"key":"2021112213003379200_B17","first-page":"376","article-title":"Enzyme Reaction Mechanism","author":"Walsh","year":"1979"},{"key":"2021112213003379200_B18","doi-asserted-by":"crossref","first-page":"3482","DOI":"10.1021\/bi00686a030","article-title":"Mechanistic studies on the rat kidney flavoenzyme L-\u03b1-hydroxy acid oxidase","volume":"14","author":"Cromartie","year":"1975","journal-title":"Biochemistry"},{"key":"2021112213003379200_B19","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0926-6569(63)90229-3","article-title":"The lactic dehydrogenase of yeast. III. D(\u2212)Lactic cytochrome c reductase, a zinc-flavoprotein from aerobic yeast","volume":"67","author":"Gregolin","year":"1963","journal-title":"Biochim. Biophys. Acta"},{"key":"2021112213003379200_B20","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1016\/S0021-9258(18)91338-4","article-title":"The lactic dehydrogenases of yeast. V. Chemical properties and function of the zinc component of D-lactic cytochrome reductase","volume":"239","author":"Cremona","year":"1964","journal-title":"J. Biol. Chem."},{"key":"2021112213003379200_B21","doi-asserted-by":"crossref","first-page":"16872","DOI":"10.1016\/S0021-9258(18)37472-6","article-title":"Isolation and characterization of a hydroxyacid-oxoacid transhydrogenase from rat kidney mitochondria","volume":"263","author":"Kaufman","year":"1988","journal-title":"J. Biol. Chem."},{"key":"2021112213003379200_B22","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/S0022-510X(96)00179-7","article-title":"2-Hydroxyglutaric aciduria: a case report on an infant with the D-isomeric form with review of the literature","volume":"143","author":"Geerts","year":"1996","journal-title":"J. Neurol. Sci."},{"key":"2021112213003379200_B23","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0009-8981(95)06074-N","article-title":"Identification of the D-enantiomer of 2-hydroxyglutaric acid in glutaric aciduria type II","volume":"238","author":"Watanabe","year":"1995","journal-title":"Clin. Chim. Acta"},{"key":"2021112213003379200_B24","first-page":"2357","article-title":"Defects of electron transfer flavoprotein and electron transfer flavoprotein-ubiquinone oxidoreductase: glutaric acidemia type II","volume-title":"The Metabolic and Molecular Bases of Inherited Disease","author":"Frerman","year":"2001","edition":"8th edn"},{"key":"2021112213003379200_B25","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.1042\/bj0312080","article-title":"The oxidation of L(\u2212)\u03b1-hydroxyglutaric acid in animal tissues","volume":"31","author":"Weil-Malherbe","year":"1937","journal-title":"Biochem. J."},{"key":"2021112213003379200_B26","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/BF02312543","article-title":"L-2-Hydroxyglutaric aciduria: an inborn error of metabolism?","volume":"3","author":"Duran","year":"1980","journal-title":"J. Inherit. Metab. Dis."}],"container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/381\/1\/35\/716555\/bj3810035.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/381\/1\/35\/716555\/bj3810035.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T08:01:59Z","timestamp":1637568119000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/381\/1\/35\/41476\/Identification-of-a-dehydrogenase-acting-on-D-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6,22]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20031933","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6,22]]}}}