{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:56:21Z","timestamp":1790675781609,"version":"4.1.0"},"reference-count":46,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,8,15]]},"abstract":"<jats:p>Insects express arthro-series glycosphingolipids, which contain an \u03b11,4-linked GalNAc residue. To determine the genetic basis for this linkage, we cloned a cDNA (CG17223) from Drosophila melanogaster encoding a protein with homology to mammalian \u03b11,4-glycosyltransferases and expressed it in the yeast Pichia pastoris. Culture supernatants from the transformed yeast were found to display a novel UDP-GalNAc:GalNAc\u03b21,4GlcNAc\u03b21-R \u03b1-N-acetylgalactosaminyltransferase activity when using either a glycolipid, p-nitrophenylglycoside or an N-glycan carrying one or two terminal \u03b2-N-acetylgalactosamine residues. NMR and MS in combination with glycosidase digestion and methylation analysis indicate that the cloned cDNA encodes an \u03b11,4-N-acetylgalactosaminyltransferase. We hypothesize that this enzyme and its orthologues in other insects are required for the biosynthesis of the N5a and subsequent members of the arthro-series of glycolipids as well as of N-glycan receptors for Bacillus thuringiensis crystal toxin Cry1Ac.<\/jats:p>","DOI":"10.1042\/bj20040535","type":"journal-article","created":{"date-parts":[[2004,8,10]],"date-time":"2004-08-10T08:40:56Z","timestamp":1092127256000},"page":"67-74","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The <i>Drosophila melanogaster<\/i> homologue of the human histo-blood group Pk gene encodes a glycolipid-modifying \u03b11,4-<i>N<\/i>-acetylgalactosaminyltransferase"],"prefix":"10.1042","volume":"382","author":[{"given":"J\u00e1n","family":"MUCHA","sequence":"first","affiliation":[{"name":"Chemick\u00fd \u00fastav, Slovensk\u00e1 akad\u00e9mia vied, D\u00fabravsk\u00e1 cesta 9, 845 38 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u0159\u00ed","family":"DOMLATIL","sequence":"additional","affiliation":[{"name":"Department f\u00fcr Chemie, Universit\u00e4t f\u00fcr Bodenkultur, Muthgasse 18, A-1190 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnter","family":"LOCHNIT","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Biochemie, Justus-Liebig-Universit\u00e4t Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dubravko","family":"RENDI\u0106","sequence":"additional","affiliation":[{"name":"Department f\u00fcr Chemie, Universit\u00e4t f\u00fcr Bodenkultur, Muthgasse 18, A-1190 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"PASCHINGER","sequence":"additional","affiliation":[{"name":"Department f\u00fcr Chemie, Universit\u00e4t f\u00fcr Bodenkultur, Muthgasse 18, A-1190 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"HINTERK\u00d6RNER","sequence":"additional","affiliation":[{"name":"Department f\u00fcr Chemie, Universit\u00e4t f\u00fcr Bodenkultur, Muthgasse 18, A-1190 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"HOFINGER","sequence":"additional","affiliation":[{"name":"Department f\u00fcr Chemie, Universit\u00e4t f\u00fcr Bodenkultur, Muthgasse 18, A-1190 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"KOSMA","sequence":"additional","affiliation":[{"name":"Department f\u00fcr Chemie, Universit\u00e4t f\u00fcr Bodenkultur, Muthgasse 18, A-1190 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain\u00a0B.\u00a0H.","family":"WILSON","sequence":"additional","affiliation":[{"name":"Department f\u00fcr Chemie, Universit\u00e4t f\u00fcr Bodenkultur, Muthgasse 18, A-1190 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2004,8,10]]},"reference":[{"key":"2021112217073872300_B1","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S0300-9084(01)01297-4","article-title":"Genetic model organisms in the study of N-glycans","volume":"83","author":"Altmann","year":"2001","journal-title":"Biochimie"},{"key":"2021112217073872300_B2","doi-asserted-by":"crossref","first-page":"15152","DOI":"10.1074\/jbc.M909620199","article-title":"Molecular cloning of globotriaosylceramide\/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids","volume":"275","author":"Kojima","year":"2000","journal-title":"J. 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