{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:30:07Z","timestamp":1791325807728,"version":"4.3.1"},"reference-count":46,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,12,1]]},"abstract":"<jats:p>Purified human mucins from different parts of the intestinal tract (ileum, cecum, transverse and sigmoid colon and rectum) were isolated from two individuals with blood group ALeb (A-Lewisb). After alkaline borohydride treatment the released oligosaccharides were structurally characterized by nano-ESI Q-TOF MS\/MS (electrospray ionization quadrupole time-of-flight tandem MS) without prior fractionation or derivatization. More than 100 different oligosaccharides, with up to ten monosaccharide residues, were identified using this technique. Oligosaccharides based on core 3 structures, GlcNAc(\u03b21-3)GalNAc (where GlcNAc is N-acetyl-D-glucosamine and GalNAc is N-acetylgalactosamine), were widely distributed in human intestinal mucins. Core 5 structures, GalNAc(\u03b11-3)GalNAc, were also recovered in all fractions. Moreover, a comparison of the oligosaccharide repertoire, with respect to size, diversity and expression of glycans and terminal epitopes, showed a high level of mucin-specific glycosylation: highly fucosylated glycans, found specifically in the small intestine, were mainly based on core 4 structures, GlcNAc-(\u03b21-3)[GlcNAc(\u03b21-6)]GalNAc, whereas the sulpho-LeX determinant carrying core 2 glycans, Gal(\u03b21-3)[GlcNAc(\u03b21-6)]-GalNAc (where Gal is galactose), was recovered mainly in the distal colon. Blood group H and A antigenic determinants were present exclusively in the ileum and cecum, whereas blood group Sda\/Cad related epitopes, GalNAc(\u03b21-4)[NeuAc(\u03b12-3)]Gal (where NeuAc is N-acetylneuraminate), were found to increase along the length of the colon. Our findings suggest that mucins create an enormous repertoire of potential binding sites for micro-organisms that could explain the regio-specific colonization of bacteria in the human intestinal tract.<\/jats:p>","DOI":"10.1042\/bj20040605","type":"journal-article","created":{"date-parts":[[2004,11,24]],"date-time":"2004-11-24T02:33:30Z","timestamp":1101263610000},"page":"307-316","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":348,"title":["Structural diversity and specific distribution of O-glycans in normal human mucins along the intestinal tract"],"prefix":"10.1042","volume":"384","author":[{"given":"Catherine","family":"ROBBE","sequence":"first","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche CNRS\/USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Universit\u00e9 des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calliope","family":"CAPON","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche CNRS\/USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Universit\u00e9 des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France"},{"name":"Laboratoire de Biochimie, Facult\u00e9 des Sciences Pharmaceutiques et Biologiques de Lille 2, 59006 Lille Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernadette","family":"CODDEVILLE","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche CNRS\/USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Universit\u00e9 des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Claude","family":"MICHALSKI","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche CNRS\/USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Universit\u00e9 des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2004,11,23]]},"reference":[{"key":"2021112217053475800_B1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0968-0004(83)90166-4","article-title":"Mucus \u2013 a protective secretion of complexity","volume":"8","author":"Allen","year":"1983","journal-title":"Trends Biochem. 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