{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:18:38Z","timestamp":1773299918140,"version":"3.50.1"},"reference-count":39,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,9,1]]},"abstract":"<jats:p>GTN (nitroglycerin; glycerol trinitrate) causes dilation of blood vessels via activation of nitric oxide (NO)-sensitive sGC (soluble guanylate cyclase), a heterodimeric haem protein that catalyses the conversion of GTP into cGMP. Activation of sGC by GTN requires enzymatic or non-enzymatic bioactivation of the nitrate. Based on insufficient NO release and lack of spectroscopic evidence for formation of NO\u2013sGC, the cysteine (Cys)-dependent activation of sGC by GTN was proposed to occur in an NO-independent manner. This extraordinary claim is questioned by the present findings. First, the effect of GTN\/Cys was blocked by the NO scavenger oxyhaemoglobin, the superoxide-generating compound flavin mononucleotide and the haem-site sGC inhibitor ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one). Secondly, at equi-effective concentrations, GTN\/Cys and the NO donor 2,2-diethyl-1-nitroso-oxyhydrazine released identical amounts of NO. Finally, at sufficiently high rates of NO release, activation of sGC by GTN\/Cys was accompanied by a shift of the Soret band from 431 to 399\u00a0nm, indicating formation of NO\u2013sGC. In the absence of Cys, GTN caused haem oxidation, apparent as a shift of the Soret band to 392\u00a0nm, which was accompanied by inactivation of the NO-stimulated enzyme. These results suggest that the effect of GTN\/Cys is the result of an activation\/inactivation equilibrium that is controlled by the rate of NO release and haem oxidation.<\/jats:p>","DOI":"10.1042\/bj20050565","type":"journal-article","created":{"date-parts":[[2005,8,23]],"date-time":"2005-08-23T15:32:31Z","timestamp":1124811151000},"page":"625-631","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of nitroglycerin\/<scp>L<\/scp>-cysteine on soluble guanylate cyclase: evidence for an activation\/inactivation equilibrium controlled by nitric oxide binding and haem oxidation"],"prefix":"10.1042","volume":"390","author":[{"given":"Antonius\u00a0C.\u00a0F.","family":"Gorren","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Karl-Franzens-Universit\u00e4t Graz, A-8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Russwurm","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Ruhr-Universit\u00e4t Bochum, D-44780 Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Kollau","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Karl-Franzens-Universit\u00e4t Graz, A-8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doris","family":"Koesling","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Ruhr-Universit\u00e4t Bochum, D-44780 Bochum, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Karl-Franzens-Universit\u00e4t Graz, A-8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Mayer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Karl-Franzens-Universit\u00e4t Graz, A-8010 Graz, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2005,8,23]]},"reference":[{"key":"2021112217183391000_B1","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1161\/01.RES.0000082524.34487.31","article-title":"Regulation of nitric oxide-sensitive soluble guanylyl cyclase","volume":"93","author":"Friebe","year":"2003","journal-title":"Circ. 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